Macro-Prep^® Cation Exchange Media

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Overview

Property	DEAE	High Q	High S	CM
Type of support	Weak anion	Strong anion	Strong cation	Weak cation
Functional ligand	–N⁺(C₂H₅)₂	–N⁺(CH₃)₃	–SO₃^-	–COO^-
Ionic capacity	175 ±75 µeq/ml	400 ±75 µeq/ml	160 ±40 µeq/ml	210 ±40 µeq/ml
Typcial binding capacity	35 mg BSA/ml	40 mg BSA/ml	70 mg IgG*/ml	35 mg hemoglobin/ml
Counterion	Cl^-	Cl^-	NA⁺	NA⁺
Nominal particle size	50 µm	50 µm	50 µm	50 µm
Nominal pore size	1,000 Å	1,000 Å	1,000 Å	1,000 Å
Recommended maximum linear flow rate	3,000 cm/hr	3,000 cm/hr	3,000 cm/hr	3,000 cm/hr
Chemical stability
1% SDS, 24 hr	Yes	Yes	Yes	Yes
6 M guanidine-HCl, 24 hr	Yes	Yes	Yes	Yes
Volume changes
pH 4–10	<1%	<1%	<3%	<1%
0.1–1.0 M NaCl	<5%	<5%	<9%	<4%
Autoclavability (121°C, 80 min)	Yes	Yes	Yes	Yes
pH stability	1–10	1–10	1–12	1–12
Antimicrobial agent	20% ethanol	20% ethanol	20% ethanol	20% ethanol
Regeneration	1 M NaCl	1 M NaCl	1 M NaCl	1 M NaCl

* Human IgG.

Macro-Prep High S strong cation exchange support meets the demands of analytical, semipreparative, and process-scale applications. This rigid, macroporous hydrophilic support provides exceptional capacity, resolution, and throughput in a chemically and mechanically stable form.

All Macro-Prep media operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength. These media provide consistent and reproducible results.

Key Features

High capacity for biomolecules
High resolution of complex biological mixtures
Rigid polymer matrix that allows high flow rates at moderate pressures
Hydrophilic matrix that reduces nonspecific binding
Macroporous beads that enhance access to ionic sites
Chemical, mechanical, and thermal stability