VersaDoc™ imaging systems are high-quality, flexible instruments that allow imaging of a wide range of samples — from 1-D and 2-D gels to chemiluminescent blots, microplates, and autoradiograms.
A Wide Range of Supported Applications
- Proteomic and genomic studies
- Protein gels (2-D), including DIGE and other multichannel differential electrophoresis techniques
- Nucleic acid and protein gel imaging (1-D)
- Multiplex gel and blot imaging
- Western, northern, and Southern blot imaging
- Quantum dot imaging
- Colony counting
The standard configuration includes a supersensitive, deeply cooled CCD camera, darkroom, power supply, cables, epi-illumination (blue and white), transillumination (UV), fluorescent reference plate, focusing target, and Quantity One® 1-D analysis software. Optional accessories include green and red excitation sources for increased multiplexing capabilities (useful for DIGE and imaging of quantum dots and organic fluroscent secondary antibodies). Two VersaDoc systems are available: the VersaDoc MP 4000 system and the VersaDoc MP 5000 system.
The VersaDoc MP 4000 system is an ideal imager for proteomic studies, offering maximum flexibility with the ability to image colorimetric and fluorescently stained gels and blots to detect chemiluminescent and other low-light and multiplex or multifluorescent samples. It resolves the finest details of every band or spot. Features include:
- 3.2 megapixel CCD with 53 µm resolution
- Accurate and quantifiable collection of data with a CV ≤5%
- Identification of differences in protein expression using optional PDQuest™ 2-D analysis software
The VersaDoc MP 5000 system is the ideal luminescent imager; it uses a back-thinned, blue-enhanced CCD with high quantum efficiency. Each camera is cooled to absolute temperatures to minimize background noise, enhance the signal-to-noise ratio, and maintain reproducible imaging conditions. This high-performance digital camera produces luminescent images of very faint samples (due to either low-abundance samples or faint signal) at speeds faster than exposure times of film-based detection. Features include:
- Supercooling to -35°C (absolute) for optimal imaging with low-light applications
- True 16-bit data and a dynamic range that covers 5 orders of magnitude to maximize limit of detection
- Quantitation of differences in sample abundance using Quantity One 1-D analysis software
- Red, green, blue, and UV illumination for multiplex quantitative imaging.
