Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep High S Cartridges

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Overview

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Bio-Scale Mini Macro-Prep High Q, High S, and DEAE cartridges are 1 ml and 5 ml Bio-Scale Mini cartridges filled with either Macro-Prep High Q, Macro-Prep High S, or Macro-Prep DEAE support:

  • High Q — strong anion exchange; chemical form –N+(CH3)3, protein capacity ≥37 mg/ml BSA
  • High S — strong cation exchange; chemical form –SO3-, the ion-moderated partition chromatography technique, protein capacity ≥49 mg/ml human IgG
  • DEAE — weak anion exchange; chemical form –HN+(C2H5)2, protein capacity >35 mg/ml protein

Macro-Prep ion exchange media meet the demands of analytical applications. These rigid, macroporous hydrophilic media provide exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep media operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength. These media provide consistent and reproducible results.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

* U.S.patent 7,208,087.

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.  
Bio-Scale Mini Macro-Prep High Q Cartridges

732-4120
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $190.00
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Bio-Scale Mini Macro-Prep High Q Cartridge

732-4122
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $75.00
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Bio-Scale Mini Macro-Prep High Q Cartridges

732-4124
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $265.00
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Bio-Scale Mini Macro-Prep High S Cartridges

732-4130
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $190.00
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Bio-Scale Mini Macro-Prep High S Cartridge

732-4132
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $75.00
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Bio-Scale Mini Macro-Prep High S Cartridges

732-4134
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $265.00
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Bio-Scale Mini Macro-Prep DEAE Cartridges

732-4140
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $210.00
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Bio-Scale Mini Macro-Prep DEAE Cartridge

732-4142
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $75.00
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Bio-Scale Mini Macro-Prep DEAE Cartridges

732-4144
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $275.00
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Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download [ Add to Cart ]
10006400 Bio-Scale Mini Macro-Prep High Q, High S, and DEAE Cartridges, 1 and 5 ml, Instruction Manual, Rev B Click to download