S1000™ Thermal Cycler

If you're like most researchers, you want to get results faster. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important — a shorter time from setup to results.

Thermal gradient feature — identifies optimal annealing temperature in a single run.

Reduced-mass sample block — fast ramping and settling produce the shortest time to target temperature available in a thermal cycler.

The patented* reduced-mass sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced.

Superior Uniformity
Precision of the temperature steps is critical for the rate and efficiency of PCR. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. The S1000 thermal cycler uses six independently controlled thermal electric modules (TEs), the heating and cooling elements of the thermal cycler, to maintain tight temperature uniformity at all points during a run — even while ramping.

Rapid Arrival at Target Temperature
A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. The S1000 thermal cycler's temperature control produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Run times can be dramatically shortened — to less than 30 min — while still producing accurate quantitative results. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs.

Superior uniformity with rapid arrival at target temperature. 1000-series thermal cyclers exhibit high average ramp rates, rapid settling time, and tight thermal uniformity throughout the ramp. This graph shows the temperature measured by probes in 15 wells across a sample block. The traces are nearly indistinguishable due to the tight uniformity. Note the consistent high average ramp rate throughout heating and cooling.

* U.S. patent 7,632,464.

Thermal Gradient for Easy Protocol Optimization

The programmable temperature gradient allows you to identify the optimal annealing temperature in a single PCR experiment by letting you test eight incubation temperatures at once.

The 1000-series gradient feature uses dynamic ramping, which means the temperature gradient forms during ramping and all wells come to the designated temperature at the same time. As a result, the incubation period is the same for all samples, which is crucial for transfer of gradient to nongradient protocols.

The O-ring hermetic seal extends the life of the heating and cooling elements. Condensation from constant heating and cooling can degrade thermal electric modules (TEs), causing them to fail. Bio-Rad’s 1000-series cyclers include a patented* O-ring seal (red in illustration) that forms an airtight barrier around the TEs, preventing exposure to condensation. This substantially lengthens the life of TEs and guarantees outstanding thermal performance.

* U.S. patent 7,051,536.

The Ultimate in Configuration Flexibility

S1000™ thermal cyclers are compatible with 96-well fast, 96–deep well, dual 48/48-well fast, and 384-well reaction modules.

	96-well fast reaction module, gradient enabled
	96–deep well reaction module, gradient enabled
	Dual 48/48-well fast reaction module, gradient enabled
	384-well reaction module, gradient enabled

Configuration Options

	Stand-Alone Stand-alone thermal cyclers and real-time PCR detections systems
	Stand-Alone Multi-Instrument C1000 Touch™ cycler control of up to 3 S1000 cyclers CFX96 Touch™ or CFX384 Touch™ system control of up to 3 S1000 cyclers
	Software-Controlled Stand-Alone C1000 Touch cycler or CFX96 Touch or CFX384 Touch system controlled by CFX Manager™ software
	Software-Controlled Multi-Instrument Up to 8 C1000 Touch cyclers, each connected to 1–3 S1000 cyclers, controlled by CFX Manager software 1 CFX96 Touch or CFX384 Touch system connected to 1–3 S1000 cyclers, controlled by CFX Manager software Up to 4 CFX96 Touch and/or CFX384 Touch systems controlled by CFX Manager software