S3™ Cell Sorter

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S3 Cell Sorter

Overview

Data Analysis on the S3 Cell Sorter Sort Data
Data Analysis on the S3™ Cell Sorter

Performance

Performance

HeLa cells were transfected with GFP and sorted by forward and side scatter, singlets, and GFP expression. A–B, presort analysis of GFP-positive cells; C–D, postsort population was sorted to >98% purity. Postsort analysis revealed that a pure GFP-positive population was successfully sorted.


Sensitivity of fluorescent microspheres

Sensitivity of fluorescent microspheres

SPHERO rainbow calibration particles (8 peaks) were excited by both 488 nm and 561 nm lasers. Emission was measured by all four detectors. Between peaks, all channels displayed a coefficient of variation (CV) <2.5 units. The FL3 channel displays the seven lowest peaks. The eighth peak is offscale due to the brightness of the signal intensity.


Cleaning system

Cleaning System

Carryover between samples is minimized using the “lock and load” backflushing system. FITC-labeled beads were run on the instrument (A). Subsequently, PE-labeled beads were run (B), followed by a repeated cycle of FITC-labeled beads (C) and PE-labeled beads (D). Each time, carryover of fluorescently labeled beads was analyzed and showed minimal carryover between samples.

Automated Drop Delay Measurement and Monitoring on the S3 Cell Sorter ProDrop™ Technology
Automated Drop Delay Measurement and Monitoring on the S3™ Cell Sorter

Optimal Cell Sorting Performance
and Purity

Obtaining optimal sort purity and maximizing recovery requires accurate drop delay determination, or measurement of the distance between the laser point of analysis and the last connected drop.

Historically, the drop delay process is labor intensive and requires bead counting on a microscope or image processing with a camera.

ProDrop™ technology automates drop delay determination and accurately calculates the drop delay value, resulting in less opportunity for error and leading to better recovery. The exact drop delay value is calculated by measuring the number of ProLine™ calibration beads in the waste stream during the QC process.

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The S3 cell sorter is the first truly walk-up automated cell sorter available to scientists. Expertly engineered with revolutionary precision and automation, the S3 cell sorter is a benchtop cell sorter equipped with one or two lasers and up to four fluorescent detectors plus scatter detectors utilizing traditional jet-in-air technology. Samples can be sorted at high speeds while maintaining sensitivity and high purity. Cell sorting has never been easier for the most common sorting experiments. Small in footprint, the S3 cell sorter brings affordable, dependable, and simplified cell sorting to every researcher.

Key Features and Benefits

  • Walk-up cell sorting — provides automation to simplify setup and optimization
  • Automated setup — creates precision sorting without user input or constant monitoring; fully automated drop delay calculation, stream-to-laser alignment, droplet break-off monitoring, and feedback ensure sort accuracy
  • Benchtop footprint — includes space-saving, internalized fluidics and temperature control system to fit in any lab
  • High speed and high purity — sorts cells faster while maintaining high sensitivity and yield
  • 2-way cell sorting — sorts 2 different defined populations at the same time
  • Automatic cleaning — reduces carryover with a dual-position loading stage that ensures the sample line is clean between sort runs

Automation

By automating the complex presort setup with unique technology, the S3 cell sorter creates a more efficient workflow. With state-of-the art technology for automated drop delay calculation, start sorting experiments with ease and accuracy with these automated features:

  • Startup/shutdown
  • Stream-to-laser alignment
  • Drop delay calculation
  • Droplet break-off monitoring and feedback
  • Phase and deflection adjustments to sort stream
  • Daily QC reporting
  • Collection volume monitoring to prevent overfilling

With an onboard monitoring and quality control system, researchers can be assured that their cells are sorted accurately for the highest purity and yield. The S3 cell sorter provides consistent and dependable sorting setups so each sort can be done with confidence.

Acquisition rate, events/sec Up to 100,000
Sorting rate, events/sec Up to 30,000
Sorting purity >99% pure
Nozzle size, µm 100
Sorting direction 2-way sorting
Sorting collection Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers Primary: 488 nm 100 mW
Optional: 561 nm 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescent detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity, MESF <125 for FITC and PE
Temperature range, °C Sample and collection temperature control system: 4–37
Peltier solid state system
Fluidics Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format FCS 3.1
Dimensions (W x D x H), ft/cm 2.3 x 2.1 x 2.1 / 70 x 65 x 65
S3™ Cell Sorter

145-1001
Cell sorter, 488 nm 100 mW laser, includes 2 fluorescent detectors with filters, 2 fluidic containers with connectors, software

List Price:   Inquire
Quantity:  
 
S3™ Cell Sorter

145-1002
Cell sorter, 488 and 561 nm 100 mW lasers, includes 4 fluorescent detectors with filters, 2 fluidic containers with connectors, software

List Price:   Inquire
Your Price:   Log in
Quantity:   Add to Cart
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Number Description Options
6325 S3™ Cell Sorter Brochure, Rev B Click to download
6329 S3 Cell Sorter Flier, Rev A Click to download
10031105 Instruction Manual, S3 Cell Sorter, Rev A Click to download
10032007 S3 Cell Sorter Quick Guide, Rev B Click to download