iTaq DNA polymerase outperforms a chemically modified hot-start DNA polymerase over a wide range of targets. Human genomic DNA fragments from 300 bp to 4 kb were amplified with both polymerases. Lane M, EZ Load 500 bp molecular ruler.
iTaq DNA polymerase performs better than other polymerases, even at low target concentrations. A 510 bp target in human genomic DNA was amplified with iTaq DNA polymerase or another supplier's hot-start DNA polymerase according to manufacturers' recommendations. Lane M, 100 bp EZ Load molecular ruler.
The high sensitivity of iTaq DNA polymerase translates to higher yields per unit of enzyme. Human genomic DNA was amplified with iTaq DNA polymerase or another supplier's hot-start DNA polymerase according to manufacturer recommendations. Lane M, 100 bp EZ Load molecular ruler.
iTaq™ DNA polymerase is an antibody-mediated hot-start DNA polymerase suitable for both PCR and real-time PCR applications. The enzyme is activated after a 3min denaturation step at 95°C. iTaq DNA polymerase is highly specific, sensitive, and easy to use.
Other benefits include:
170-8870 250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2
170-8875 5,000 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 25 ml each 10x PCR buffer and 50 mM MgCl2
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