Bio-Plex Pro™ Magnetic Cell Signaling Assays

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Bio-Plex Pro Magnetic Cell Signaling Assays

Overview

Available Magnetic Assays

Phosphoprotein Assays   Total Protein Target Assays Bead Region
Akt (Ser473) 171-V50001M Akt 171-V60001M 75
Akt (Thr308) 171-V50002M
c-Jun (Ser63) 171-V50003M c-Jun 171-V60002M 56
EGFR (Tyr1068) 171-V50004M     44
EGFR (Tyr1173) 171-V50005M     44
Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) 171-V50006M Erk 1/2 171-V60003M 38
GSK-3α/β (Ser21/Ser9) 171-V50007M GSK-3β 171-V60004M 18
HER-2 (Tyr1248) 171-V50008M HER-2 171-V60005M 30
IGF-IR (Tyr1131) 171-V50009M     43
IκB-α (Ser32/Ser36) 171-V50010M IκB-α 171-V60006M 67
JNK (Thr183/Tyr185) 171-V50011M JNK 171-V60007M 34
MEK1 (Ser217/Ser221) 171-V50012M MEK1 171-V60008M 27
NF-κB p65 (Ser536) 171-V50013M     37
p38 MAPK (Thr180/Tyr182) 171-V50014M p38 MAPK 171-V60009M 36
p70 S6 Kinase (Thr421/Ser424) 171-V50015M p70 S6 Kinase 171-V60010M 55
p70 S6 Kinase (Thr389) 171-V50016M
PDGFR-α (Tyr754) 171-V50017M     28
PDGFR-β (Tyr751) 171-V50018M     57
Smad2 (Ser465/Ser467) 171-V50019M Smad2 171-V60011M 14
Stat1 (Tyr701) 171-V50020M     61
Stat3 (Ser727) 171-V50021M     52
Stat3 (Tyr705) 171-V50022M     52
VEGFR-2 (Tyr1175) 171-V50023M     29

 

CST antibodies developed and validated for Bio-Plex phosphoprotein and total target assays.


Multiplexability Matrix

Find assays compatible for multiplexing.

Download multiplexability tables

Bio-Plex Pro™ Cell Signaling Assays: What to Order
Bio-Plex Pro cell signaling assays and reagents are designed to provide up to 96 reactions. Create a complete assay by ordering the following components:

Bio-Plex Pro cell signaling singleplex sets* — include coupled beads and detection antibodies. Users can mix compatible sets to create multiplex assays. See the multiplexability matrix for guidance.

Bio-Plex Pro cell signaling reagent kit** — includes cell lysis buffer, cell lysis factor QG, cell wash buffer, bead resuspension buffer, detection antibody diluent, wash buffer, streptavidin-PE, 96-well flat bottom plate, sealing tape, and instructions.

Bio-Rad cell lysate controls*** — refer to the lysate selection guide to identify the appropriate lysate for your assay.

* Required for detecting your phosphoprotein or total protein of interest.

** Required for preparing lysates and running the assay.

*** Recommended for use as qualitative phosphoprotein and total protein assay controls.

Bio-Rad Cell Lysate Controls* Selection Guide

Phosphoprotein Lysate Control Catalog #
Akt (Ser473)
Akt (Thr308)
Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)
GSK-3α/β (Ser21/Ser9)
MEK1 (Ser217/Ser221)
EGF-treated HEK-293 #171-YZ0001
c-Jun (Ser63)
JNK (Thr183/Tyr185)
p38 MAPK (Thr180/Tyr182)
UV-treated HEK-293 #171-YZ0009
EGFR (Tyr1068)
EGFR (Tyr1173)
EGF-treated HeLa #171-YZ0002
HER-2 (Tyr1248) EGF-treated SK-Br3 #171-YZ0003
IGF-IR (Tyr1131) IGF-1-treated HEK-293 #171-YZ0005
IκB-α (Ser32/Ser36)
NF-κB p65 (Ser536)
Smad2 (Ser465/Ser467)
TNF-α-treated HeLa #171-YZ0008
p70 S6 Kinase (Thr421/Ser424)
p70 S6 Kinase (Thr389)
NGFβ-treated PC12 #171-YZ0006
PDGFR-α (Tyr754)
PDGFR-β (Tyr751)
PDGF-treated NIH3T3 #171-YZ0007
Stat1 (Tyr701)
Stat3 (Ser727)
Stat3 (Tyr705)
IFN-α-treated HeLa #171-YZ0004
VEGFR-2 (Tyr1175) VEGF-treated HUVEC #171-YZ0010
Negative control for
all phosphoprotein assays
Phosphatase-treated HeLa #171-YZB001
Total Target Lysate Control Catalog #
All total target assays
except HER-2 and c-Jun
Untreated HeLa #171-YZT002
Total c-Jun Untreated HEK-293 #171-YZT001
Total HER-2 EGF-treated SK-Br3 #171-YZ0003
Negative control for
all total target assays
Detection antibody diluent**  

* All controls are shipped as one vial of lyophilized cell lysate.

** Included with the Bio-Plex Pro cell signaling reagent kit (#171-304006M).

 

Bio-Plex Assay Builder

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder



Bio-Plex Pro magnetic cell signaling assays are multiplexed immunoassays for the detection of intracellular phosphoproteins and total target proteins in cell and tissue lysates. These assays utilize tailored antibodies bound on 6.5 µm magnetic beads. Bio-Plex Pro total target assays measure the total abundance of each target protein, regardless of its phosphorylation state. Bio-Plex Pro phosphoprotein assays measure the relative amounts of phosphorylated proteins only. When both phosphoprotein and total protein assays are performed, they must be run in different wells due to shared bead regions. Using the multiplex format, the profiles of multiple phosphorylated proteins involved in cell signaling pathways can be detected using only 50 µl of lysate.

Features and Benefits of Magnetic Cell Signaling Assays

  • Compatible singleplex sets can be combined to create your own multiplex assay
  • Assay protocol optimized for high sensitivity and broad dynamic range
  • Highly specific antibodies developed and validated by Cell Signaling Technology, Inc.
  • Higher specificity sandwich format that minimizes cross-reactivity with other cellular proteins
  • Low sample volume requirement
  • Minimal hands-on time
  • Assay quick guide to get you started right away

Applications and Uses of Magnetic Cell Signaling Assays

The capacity for multiplexed biomarker analysis makes the Bio-Plex® suspension array system the ideal platform for signal transduction assays. Bio-Plex Pro magnetic cell signaling technology can be used to determine intracellular levels of phosphorylated proteins in the investigation of diverse biological processes including apoptosis, translation control, gene expression, cell cycle regulation, cytoskeletal restructuring, and neurological function.

Required Reagents

In addition to a phosphoprotein or total target assay, the Bio-Plex Pro cell signaling reagent kit (#171-304006M) is needed to perform a Bio-Plex Pro cell signaling assay.

Recommended Reagents

For best results it is recommended that the appropriate cell lysate controls be included in each experiment. Refer to the Bio-Rad cell lysate controls selection guide to identify the appropriate controls for your Bio-Plex Pro cell signaling phosphoprotein or total target assay of interest.

Other Assay Formats Available

Custom assays available as Bio-Plex® x-Plex™ assays

Instrument Compatibility

More Information

Other Bio-Plex Magnetic Bead-Based Immunoassays are available for diverse fields of research:

See also:

Reactive species Human, mouse, rat
Matrices Cell culture lysate, tissue lysate
Limit of detection ≤10 µg/total protein
Precision Intra-assay CV <15%
Inter-assay CV <25%

Values vary by assay.

Bio-Plex Pro Phospho Akt (Ser473) Set (#171-V50001M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Akt (Ser473) Set

171-V50001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Akt (Ser473), requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Phospho-Akt (Thr308) Set (#171-V50002M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Akt (Thr308) Set

171-V50002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Akt (Thr308), requires reagent kit and lysates

List Price:   $275.00
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Bio-Plex Pro Phospho-c-Jun (Ser63) Set (#171-V50003M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-c-Jun (Ser63) Set

171-V50003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated c-Jun (Ser63), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) Set (#171-V50006M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) Set

171-V50006M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Erk1/2 (Thr202/Tyr204, Thr185/Tyr187), requires reagent kit and lysates

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Bio-Plex Pro Phospho-GSK-3α/ĂŸ (Ser21/Ser9) Set (#171-V50007M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-GSK-3α/β (Ser21/Ser9) Set

171-V50007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated GSK-3α/β (Ser21/Ser9), requires reagent kit and lysates

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Bio-Plex Pro Phospho-HER-2 (Tyr1248) Set (#171-V50008M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-HER-2 (Tyr1248) Set

171-V50008M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated HER-2 (Tyr1248), requires reagent kit and lysates

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Bio-Plex Pro Phospho-IGF-IR (Tyr1131) Set (#171-V50009M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-IGF-IR (Tyr1131) Set

171-V50009M
1 x 96-well, Includes coupled magnetic beads and detection antibodies for detecting phosphorylated IGF-IR (Tyr1131), requires reagent kit and lysates

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Bio-Plex Pro Phospho-I?B-a (Ser32/Ser36) Set (#171-V50010M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-IκB-α (Ser32/Ser36) Set

171-V50010M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated IκB-α (Ser32/Ser36), requires reagent kit and lysates

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Bio-Plex Pro Phospho-JNK (Thr183/Tyr185) Set (#171-V50011M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-JNK (Thr183/Tyr185) Set

171-V50011M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated JNK (Thr183/Tyr185), requires reagent kit and lysates

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Bio-Plex Pro Phospho-MEK1 (Ser217/Ser221) Set (#171-V50012M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-MEK1 (Ser217/Ser221) Set

171-V50012M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated MEK1 (Ser217/Ser221), requires reagent kit and lysates

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Bio-Plex Pro Phospho-NF-?B p65 (Ser536) Set (#171-V50013M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-NF-κB p65 (Ser536) Set

171-V50013M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated NF-κB p65 (Ser536), requires reagent kit and lysates

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Bio-Plex Pro Phospho-p38 MAPK (Thr180/Tyr182) Set (#171-V50014M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-p38 MAPK (Thr180/Tyr182) Set

171-V50014M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p38 MAPK (Thr180/Tyr182), requires reagent kit and lysates

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Bio-Plex Pro Phospho-p70 S6 Kinase (Thr421/Ser424) Set (#171-V50015M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-p70 S6 Kinase (Thr421/Ser424) Set

171-V50015M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p70 S6 Kinase (Thr421/Ser424), requires reagent kit and lysates

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Bio-Plex Pro Phospho-p70 S6 Kinase (Thr389) Set (#171-V50016M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-p70 S6 Kinase (Thr389) Set

171-V50016M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated p70 S6 Kinase (Thr389), requires reagent kit and lysates

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Bio-Plex Pro Phospho-PDGFR-a (Tyr754) Set (#171-V50017M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-PDGFR-α (Tyr754) Set

171-V50017M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated PDGFR-α (Tyr754), requires reagent kit and lysates

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Bio-Plex Pro Phospho-PDGFR-ĂŸ (Tyr751) Set (#171-V50018M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-PDGFR-β (Tyr751) Set

171-V50018M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated PDGFR-β (Tyr751), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Smad2 (Ser465/Ser467) Set (#171-V50019M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Smad2 (Ser465/Ser467) Set

171-V50019M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Smad2 (Ser465/Ser467), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Stat1 (Tyr701) Set (#171-V50020M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Stat1 (Tyr701) Set

171-V50020M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Stat1 (Tyr701), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Stat3 (Ser727) Set (#171-V50021M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Stat3 (Ser727) Set

171-V50021M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Stat3 (Ser727), requires reagent kit and lysates

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Bio-Plex Pro Phospho-Stat3 (Tyr705) Set (#171-V50022M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-Stat3 (Tyr705) Set

171-V50022M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated Stat3 (Tyr705), requires reagent kit and lysates

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Bio-Plex Pro Phospho-VEGFR-2 (Tyr1175) Set (#171-V50023M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Phospho-VEGFR-2 (Tyr1175) Set

171-V50023M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting phosphorylated VEGFR-2 (Tyr1175), requires reagent kit and lysates

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Bio-Plex Pro Total Akt Set (#171-V60001M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total Akt Set

171-V60001M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Akt, requires reagent kit and lysates

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Bio-Plex Pro Total c-Jun Set (#171-V60002M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total c-Jun Set

171-V60002M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total c-Jun, requires reagent kit and lysates

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Bio-Plex Pro Total Erk1/2 Set (#171-V60003M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total Erk1/2 Set

171-V60003M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Erk1/2, requires reagent kit and lysates

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Bio-Plex Pro Total GSK-3ĂŸ Set (#171-V60004M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total GSK-3β Set

171-V60004M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total GSK-3β, requires reagent kit and lysates

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Bio-Plex Pro Total HER-2 Set (#171-V60005M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total HER-2 Set

171-V60005M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total HER-2, requires reagent kit and lysates

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Bio-Plex Pro Total I?B-a Set (#171-V60006M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total IκB-α Set

171-V60006M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total IκB-α, requires reagent kit and lysates

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Bio-Plex Pro Total JNK Set (#171-V60007M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total JNK Set

171-V60007M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total JNK, requires reagent kit and lysates

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Bio-Plex Pro Total MEK1 Set (#171-V60008M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total MEK1 Set

171-V60008M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total MEK1, requires reagent kit and lysates

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Bio-Plex Pro Total p38 MAPK Set (#171-V60009M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total p38 MAPK Set

171-V60009M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total p38 MAPK, requires reagent kit and lysates

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Bio-Plex Pro Total p70 S6 Kinase Set (#171-V60010M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total p70 S6 Kinase Set

171-V60010M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total p70 S6 Kinase, requires reagent kit and lysates

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Bio-Plex Pro Total Smad2 Set (#171-V60011M) - Bio-Plex Pro Cell Signaling Assay
Bio-Plex Pro™ Total Smad2 Set

171-V60011M
1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting total Smad2, requires reagent kit and lysates

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Bio-Plex Pro EGF-Treated HEK293 Lysate (#171-YZ0001) - Bio-Plex Pro Cell Signaling Assay
EGF-Treated HEK-293 Lysate

171-YZ0001
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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EGF-Treated HeLa Lysate (#171-YZ0002) - Bio-Plex Pro Cell Signaling Assay
EGF-Treated HeLa Lysate

171-YZ0002
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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EGF-Treated SK-Br3 Lysate (#171-YZ0003) - Bio-Plex Pro Cell Signaling Assay
EGF-Treated SK-Br3 Lysate

171-YZ0003
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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IFN-a-Treated HeLa Lysate (#171-YZ0004) - Bio-Plex Pro Cell Signaling Assay
IFN-α-Treated HeLa Lysate

171-YZ0004
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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IGF-1-Treated HEK-293 Lysate (#171-YZ0005) - Bio-Plex Pro Cell Signaling Assay
IGF-1-Treated HEK-293 Lysate

171-YZ0005
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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NGFĂŸ-Treated PC12 Lysate (#171-YZ0006) - Bio-Plex Pro Cell Signaling Assay
NGFβ-Treated PC12 Lysate

171-YZ0006
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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PDGF-Treated NIH3T3 Lysate (#171-YZ0007) - Bio-Plex Pro Cell Signaling Assay
PDGF-Treated NIH3T3 Lysate

171-YZ0007
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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TNF-a-Treated HeLa Lysate (#171-YZ0008) - Bio-Plex Pro Cell Signaling Assay
TNF-α-Treated HeLa Lysate

171-YZ0008
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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UV-Treated HEK-293 Lysate (#171-YZ0009) - Bio-Plex Pro Cell Signaling Assay
UV-Treated HEK-293 Lysate

171-YZ0009
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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VEGF-Treated HUVEC Lysate (#171-YZ0010) - Bio-Plex Pro Cell Signaling Assay
VEGF-Treated HUVEC Lysate

171-YZ0010
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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Phosphatase-Treated HeLa Lysate (#171-YZB001) - Bio-Plex Pro Cell Signaling Assay
Phosphatase-Treated HeLa Lysate

171-YZB001
Pkg of 1, lyophilized cell lysate, negative control for phosphoprotein detection methods

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Untreated HEK-293 (#171-YZT001) - Bio-Plex Pro Cell Signaling Assay
Untreated HEK-293

171-YZT001
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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Untreated HeLa Lysate (#171-YZT002) - Bio-Plex Pro Cell Signaling Assay
Untreated HeLa Lysate

171-YZT002
Pkg of 1, lyophilized cell lysate, positive control for phosphoprotein or total target protein detection methods

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Number Description Options
6285 Bio-Plex Pro Cell Signaling Assays Product Information Sheet, Rev A Click to download [ Add to Cart ]
6335 Bio-Plex Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev B Click to download
5507 Bio-Plex Multiplex System Ordering Information Sheet, Interactive PDF, Rev K Click to download
10024929 Instruction Manual, Bio-Plex Pro Cell Signaling Assays, Rev A Click to download
10024930 Instruction Manual, Bio-Plex Pro Assays, Quick Guide 3, Rev A Click to download
6330 Profiling of Cell Signaling Pathways and Angiogenesis Biomarkers in Human Cancer Tissue Click to download
6331 Validation and Application of Bio-Plex Pro Cell Signaling Assays Click to download