LF PVDF membranes have lower autofluorescence than traditional PVDF membranes. Membranes were scanned dry on a VersaDoc™ MP 5000 system in blue excitation for 5 sec, green excitation for 5 sec, and red excitation for 20 sec. Each background was determined using Image Lab™ software.
Linear signal for accurate blot quantitation. Different dilutions of an albumin-depleted human serum sample were loaded on a Criterion™ TGX™ 4–20% gel and transferred to a low fluorescence PVDF membrane using tank blotting. The primary antibody used was rabbit anti-transferrin antibody and the secondary antibody was donkey anti-rabbit Alexa Fluor 568. The membrane was scanned using a VersaDoc™ MP 5000 system. Quantitation of the band was performed using Image Lab™ software.
Bio-Rad imager family
Multiplex detection of two targets simultaneously. Albumin-depleted human serum samples spiked with STI and Profinia™ GST extract run on a Criterion™ TGX™ 4–20% stain-free gel, transferred to a membrane of choice, and blotted. The primary antibodies used were goat anti-GST and rabbit anti-STI. The secondary antibodies used were anti-rabbit Alexa Fluor 546 and anti-goat Alexa Fluor 488. The membranes were scanned using a VersaDoc™ MP 5000 system.
LF PVDF membranes are available precut for mini or midi gel sizes in 10- or 20-pack configurations, or as a large roll.
For more information, download bulletins 1939 and 2212.
Immun-Blot® low fluorescence PVDF membrane offers the same benefits as standard PVDF membranes in addition to minimal autofluorescence and high sensitivity, making it the premium choice for multiplex fluorescent immunoblotting needs.
Features and Benefits
Applications
Please refer to the Immun-Blot Low Fluorescence PVDF brochure for more information and data.
See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.
162-0260 Pkg of 10, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting
162-0261 Pkg of 20, 7 x 8.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting
162-0262 Pkg of 10, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting
162-0263 Pkg of 20, 8.5 x 13.5 cm, precut low fluorescence PVDF/filter paper for immunoblotting
162-0264 Pkg of 1 roll, 28 cm x 3.8 m, low fluorescence PVDF membrane for immunoblotting
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