Bio-Plex Pro™ Human Cytokine, Chemokine, and Growth Factor Assays

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Overview

Available Human Assays
Available Bio-Plex Pro™ Human Cytokine Assays
Human Group I
8-Plex Panel (M50-000007A)
17-Plex Panel (M50-00031YV)
27-Plex Panel (M50-0KCAF0Y)
Th1/Th2 Panel (M50-00005L3)
Singleplex/ x-Plex Bead Regions*
IL-1β
 
• 
• 
  
39
IL-1rα    
• 
  
25
IL-2
• 
• 
• 
• 
38
IL-4
• 
• 
• 
• 
52
IL-5  
• 
• 
• 
33
IL-6
• 
• 
• 
  
19
IL-7  
• 
• 
  
74
IL-8
• 
• 
• 
  
54
IL-9    
• 
  
77
IL-10
• 
• 
• 
• 
56
IL-12 p70  
• 
• 
• 
75
IL-13  
• 
• 
• 
51
IL-15    
• 
  
73
IL-17  
• 
• 
  
76
Basic FGF    
• 
  
44
Eotaxin    
• 
  
43
G-CSF  
• 
• 
  
57
GM-CSF
• 
• 
• 
• 
34
IFN-γ
• 
• 
• 
• 
21
IP-10    
• 
  
48
MCP-1 (MCAF)
  
• 
• 
  
53
MIP-1α    
• 
  
55
MIP-1β  
• 
• 
  
18
PDGF-BB    
• 
  
47
RANTES    
• 
  
37
TNF-α
• 
• 
• 
• 
36
VEGF    
• 
  
45

 

Human Group II
21-Plex Panel
(MF0-005KMII)
Singleplex/ x-Plex Bead Regions*
IL-1α
• 
63
IL-2Rα
• 
13
IL-3
• 
64
IL-12 (p40)
• 
28
IL-16
• 
27
IL-18**
• 
42
CTACK
• 
72
GRO-α
• 
61
HGF
• 
62
ICAM-1
 
12
IFN-α2
• 
20
LIF
• 
29
MCP-3
• 
26
M-CSF
• 
67
MIF
• 
35
MIG
• 
14
β-NGF
• 
46
SCF
• 
65
SCGF-β
• 
78
SDF-1α
• 
22
TNF-β
• 
30
TRAIL
• 
66
V-CAM1  
15

 

Th17 Assays
15-Plex Panel
(171-AA001M)
Singleplex
Bead Regions
IL-1β
• 
39
IL-4
• 
52
IL-6
• 
19
IL-10
• 
56
IL-17A
• 
76
IL-17F
• 
44
IL-21
• 
47
IL-22
• 
18
IL-23
• 
43
IL-25
• 
55
IL-31
• 
62
IL-33
• 
46
IFN-γ
• 
21
sCD40L
• 
29
TNF-α
• 
36
IL-17A/F***  
66

 

TGF-β Group†
3-Plex Panel
(171-W4001M)
Singleplex
Bead Regions
TGF-β1
• 
13
TGF-β2
• 
72
TGF-β3
• 
66

 

* Custom assays can be ordered with any combination of analytes within a group, except for ICAM-1 and VCAM-1, which require different sample dilutions. For more information about compatibility between groups see bulletin 5927.

** IL-18 is not available as a singleplex assay, only as part of an assay panel.

*** IL-17A/F is available only as a singleplex set and cannot be mixed with IL-17A or IL-17F.

† TGF-β assays are compatible with human, mouse, and rat samples.

 

Specifications Specifications
Bio-Plex Pro™ Human Cytokine Assay Specifications

Representative assay working ranges,* assay sensitivity, and precision.** The LLOQ, ULOQ, LOD, and intra-/inter-assay %CV are mean data determined from five assays in serum-based matrix.

  Assay Working Ranges, pg/ml Assay Sensitivity, pg/ml Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Group I 27-plex panel
IL-1β 3.2 3,261 0.6 6 8
IL-1rα 81.1 70,487 5.5 9 9
IL-2 2.1 17,772 1.6 7 9
IL-4 2.2 3,467 0.7 9 8
IL-5 3.1 7,380 0.6 8 10
IL-6 2.3 18,880 2.6 7 11
IL-7 3.1 6,001 1.1 6 8
IL-8 1.9 26,403 1.0 9 4
IL-9 2.1 7,989 2.5 8 9
IL-10 2.2 8,840 0.3 5 6
IL-12 (p70) 3.3 13,099 3.5 6 6
IL-13 3.7 3,137 0.7 8 7
IL-15 2.1 2,799 2.4 5 6
IL-17 4.9 12,235 3.3 8 6
Eotaxin 40.9 5,824 2.5 8 11
Basic FGF 27.2 7,581 1.9 8 8
G-CSF 2.4 11,565 1.7 10 5
GM-CSF 63.3 6,039 2.2 12 6
IFN-γ 92.6 52,719 6.4 15 9
IP-10 18.8 26,867 6.1 11 9
MCP-1 2.1 1,820 1.1 9 7
MIP-1α 1.4 836 1.6 7 8
MIP-1β 2.0 1,726 2.4 8 8
PDGF-BB 7.0 51,933 2.9 9 8
RANTES 2.2 8,617 1.8 9 6
TNF-α 5.8 95,484 6.0 8 6
VEGF 5.5 56,237 3.1 9 7
Group II 21-plex panel, ICAM-1 and VCAM-1
CTACK 13 16,874 3.4 5 6
GRO-α 19 2,711 6.3 5 8
HGF 9.5 25,357 4.9 5 6
IFN-α2 307 3,438 4.3 7 3
IL-1α 1.4 22,569 0.5 4 4
IL-2Rα 299 7,338 2.1 6 4
IL-3 12 22,803 4.8 7 4
IL-12 (p40) 41 28,662 23.3 5 8
IL-16 190 12,133 0.4 6 4
IL-18 1.8 28,677 0.2 4 5
LIF 12 22,184 5.5 4 3
MCP-3 79 11,234 1.0 7 8
M-CSF 2.0 32,200 0.9 4 5
MIF 231 24,373 1.5 5 7
MIG 2.7 8,584 1.2 6 6
β-NGF 1.0 4,247 0.2 4 7
SCF 1.5 25,078 1.0 5 4
SCGF-β 66 47,862 45.4 6 8
SDF-1α 10 10,391 8.7 6 6
TNF-β 1.5 24,505 0.3 4 4
TRAIL 4.4 44,769 2.1 8 8
 
ICAM-1 13 26,368 2.4 4.3 3.8
VCAM-1 38 21,430 0.6 6.7 5.5

* Assay range is LLOQ and ULOQ, calculated from five independent assays.

** Data were generated using vacuum manifold. %CV is expected to be comparable or lower with magnetic bead washer.

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

TGF-β Assay Specifications
TGF-β Assay Specifications Overlay
Incubation time
3-Plex Panel
(171-W4001)
Sample volume
12.5 µl
Accuracy (recovery range)
80–100%
Precision
   Intra-assay %CV
   Inter-assay %CV
   ≤10%
   ≤15%


Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–100%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

TGF-β Assay Specifications
 
Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Premixed 3-Plex Assay
TGF-β1 1.69  27,616 3.9
4.5
 4.9
TGF-β2
14.7 
 30,080 1.9
6.3 
 9.1
TGF-β3
2.8 
 15,031 0.5
6.9 
 8.2


Assay Performance Definitions
Assay working range – the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ)

Precision – the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy – ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) – the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex Assay Builder
Bio-Plex Assay Builder

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder



Bio-Plex Pro™ human cytokine, chemokine, and growth factor magnetic bead–based assays detect and measure levels of 53 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth, to infections, to Parkinson's disease.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • Compatible with Bio-Plex Pro human diabetes assays for an expanded biomarker profile

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits (8-plex, 17-plex, 21-plex, 27-plex, Th1/Th2, TGF-β 3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-D50001 for group I cytokines, #171-D60001 for group II cytokines, or #171-X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex® x-Plex™ assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express assays built using the Bio-Plex Assay Builder

For multiplexing capabilities among cytokines, refer to bulletin 5927. For multiplexing capabilities with diabetes assays, refer to bulletin 5975.

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex 3D system
  • Luminex MAGPIX system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station

Bio-Plex Pro™ Human Cytokine 8-plex Assay #M50-000007A
Bio-Plex Pro™ Human Cytokine 8-plex Assay

M50-000007A
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 human cytokines all in one kit

List Price:   $1,470.00
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Bio-Plex Pro Human Cytokine TH1/TH2 Assay #M50-00005L3
Bio-Plex Pro Human Cytokine Th1/Th2 Assay

M50-00005L3
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 9 human cytokines all in one kit

List Price:   $1,670.00
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Bio-Plex Pro Human Cytokine 17-plex Assay #M50-00031YV
Bio-Plex Pro Human Cytokine 17-plex Assay

M50-00031YV
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 17 human cytokines all in one kit

List Price:   $2,490.00
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Bio-Plex Pro Human Cytokine 27-plex Assay #M50-0KCAF0Y
Bio-Plex Pro Human Cytokine 27-plex Assay

M50-0KCAF0Y
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 27 human cytokines all in one kit

List Price:   $3,670.00
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Bio-Plex Pro Human Cytokine 21-plex Assay #MF0-005KMII
Bio-Plex Pro Human Cytokine 21-plex Assay

MF0-005KMII
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 21 human cytokines all in one kit

List Price:   $2,930.00
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Bio-Plex Pro™ TGF-β 3-plex Assay

171-W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, sealing tape, for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

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Number Description Options
6335 Bio-Plex Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev B Click to download
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart ]
10014905 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays, Rev D Click to download [ Add to Cart ]
10024985 Instruction Manual, Bio-Plex Pro Assays Quick Guide 4, Rev A Click to download
5297 Bio-Plex Cytokine Assay References, Rev C Click to download
2993 Bio-Plex Glossary, Rev A Click to download [ Add to Cart ]
5800 Development and Validation of Two Magnetic Bead-Based Mouse Cytokine Multiplex Assay Panels, Rev A Click to download [ Add to Cart ]
5803 Development and Validation of Two Human Cytokine Multiplex Assay Panels on Magnetic Microspheres, Rev A Click to download [ Add to Cart ]
5817 Bio-Plex Pro/Bio-Plex Pro II Wash Stations: Validation for Bead-Based Immunosandwich Suspension Array Assays Tech Poster, Rev A Click to download
5828 Bio-Plex Pro Human Cytokine, Chemokine, and Growth Factor Assays Product Sheet, Rev A Click to download [ Add to Cart ]
5507 Bio-Plex Multiplex System Ordering Information Sheet, Interactive PDF, Rev K Click to download
5818 Multiplex Analysis of Serum Biomarkers in Ovarian Cancer Patients Using Bio-Plex Suspension Array System Tech Poster, Rev B Click to download
5927 Multiplexing Across Cytokine Panels, Rev A Click to download [ Add to Cart ]
5975 Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays, Rev A Click to download [ Add to Cart ]
BR125FS Second Generation Multiplex Immunoassays Click to download
6029 Normal Physiological Levels of Human Cytokines Using Bio-Plex Pro Cytokine Assays, Rev A Click to download [ Add to Cart ]
6054 Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B Click to download [ Add to Cart ]