Bio-Plex Pro™ Mouse Th17 Assays

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Bio-Plex Pro™ Mouse Th17 Assays

Overview

Th17 Assay Specifications Th17 Assay Specifications
Bio-Plex Pro™ Mouse Th17 Assay Specifications
  Assay Working Ranges pg/ml Assay Sensitivity pg/ml Assay Precision
  Target LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV
8-Plex IL-17F 1 6,362 0.1 5 5
IL-21 6 8,207 2.8 3 15
IL-22 0.5 8,433 0.1 4 5
IL-23p19 3.4 55,764 0.7 5 6
IL-31 2.5 41,313 5.2 6 6
IL-33 0.5 7,343 0.2 5 12
CD40L 3.5 11,245 0.4 4 14
MIP-3α 0.7 1,660 0.3 6 13
 
6-Plex IL-1α 10.36 60,631 9.4 4 7
IL-6 0.74 12,053 0.2 3 16
IL-10 2.95 12,066 1 4 5
IL-17A 2.65 43,337 0.8 3 10
IFN-γ 1.84 30,164 1.2 4 6
TNF-α 5.8 59,626 1.4 3 6
 
2-Plex IL-25 168 98,266 9 9 12
IL-27p28 63.4 117,521 29.2 9 6
 
Singleplex ICAM-1 2.9 23,723 0.78 4 8

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (limit of detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal

The Bio-Plex Pro™ mouse Th17 magnetic bead–based immunoassays detect and measure the levels of 17 Th17 cell–associated biomarkers in diverse matrices such as serum, plasma, and tissue culture supernatants. IL-17 producing T helper cells (Th17 cells) are a new subset of T helper cells recently found to be associated with autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, and lupus. The markers in these assays were selected for their direct relevance to the mouse Th17 pathway.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits (panel A 6-plex, panel B 8-plex, TGF-β 3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection bodies; requires a reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-I50001 for group I cytokines, #171-IA0001 for group III cytokines, or #171-X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex® x-Plex™ assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express assays built using the Bio-Plex Assay Builder

For multiplexing capabilities with other cytokines, refer to bulletin 5927. For multiplexing capabilities with diabetes assays, refer to bulletin 5975.

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex 3D system
  • Luminex MAGPIX system
  • Bio-Plex Pro wash station
  • Bio-Plex Pro II wash station
Bio-Plex Pro™ Mouse Cytokine Th17 Panel A 6-Plex Group l

M60-00007NY
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 6 mouse cytokines all in one kit

List Price:   $1,200.00
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Bio-Plex Pro™ Mouse Cytokine Th17 Panel B 8-Plex Group III

171-FA001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit

List Price:   $1,715.00
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Bio-Plex Pro™ TGF-β 3-plex Assay

171-W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, sealing tape, for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

List Price:   $845.00
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Number Description Options
6335 Bio-Plex Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev B Click to download
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart ]
6118 Analysis of Murine Th17 Cytokine Profiles using Bio-Plex Pro Mouse Th17 Panel, Rev A Click to download [ Add to Cart ]
6110 Bio-Plex Pro Mouse Th17 Assays Product Information Sheet, Rev B Click to download [ Add to Cart ]
5975 Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays, Rev A Click to download [ Add to Cart ]
5927 Multiplexing Across Cytokine Panels, Rev A Click to download [ Add to Cart ]
10014905 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays, Rev D Click to download [ Add to Cart ]
10024985 Instruction Manual, Bio-Plex Pro Assays Quick Guide 4, Rev A Click to download
2993 Bio-Plex Glossary, Rev A Click to download [ Add to Cart ]
5507 Bio-Plex Multiplex System Ordering Information Sheet, Interactive PDF, Rev K Click to download
3100 Bio-Plex Cytokine Assay Comparison, Rev E Click to download [ Add to Cart ]
5297 Bio-Plex Cytokine Assay References, Rev C Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download