The Criterion Stain Free™ gel imaging system allows direct visualization, analysis, and documentation of protein samples in SDS-PAGE gels with the single touch of a button, without staining, destaining, or gel drying procedures. The complete system is composed of the Criterion Stain Free imager*, Criterion Stain Free gels, and Image Lab™ software. This stain-free system is ideal for quick sample assessment prior to purification procedures. The system is also useful as a precursor to blotting and profiling workflows in which Coomassie Blue staining is ordinarily used as a detection method for purity assessment, quantitation, protein characterization (western blotting), and profiling (MALDI-TOF, LC-MS/MS). Benefits include:
- Fast results — Elimination of staining, destaining, and gel drying steps shortens the electrophoresis portion of the experimental workflow, such as sample assessment and data analysis in protein purification; researchers can plan for the next purification step or for the next project
- Reproducibility — Gel imaging and analysis is automated, enabling reproducible results. There is no background variability within a gel or between gels (as is often seen with standard Coomassie Blue staining); the Criterion Stain Free system enables standardization of methods
- Green technology — Acetic acid and methanol are eliminated, reducing organic waste
- Ease of use — One-touch gel image analysis processing and data reporting is accomplished through a single user interface, reducing the number of steps to achieve results
*The Criterion Stain Free imager is no longer offered by Bio-Rad Laboratories. It has been replaced by the new Gel Doc EZ imager. However, we provide support for those who have already purchased the system in case of any problems or questions. Click here to learn more about the new system.