Zeta-Probe® membranes are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge. These pliable, flexible membranes are resistant to shrinking, tearing, and becoming brittle during transfer, hybridization, or reprobing procedures.
Zeta-Probe membranes bind nucleic acids independently of buffer pH, so they can be used in traditional Southern blots, rapid alkaline Southern or northern blotting techniques, and electrophoretic transfer of nucleic acids from agarose and polyacrylamide gels.
Zeta-Probe membranes can be repeatedly hybridized and stripped at 100°C in 0.5% SDS, 0.1x SSC as many as 20 times for DNA (Li et al. 1987) and 6 times for RNA (Gatti et al. 1984). Oligonucleotides as short as 6 bases will bind to the membrane, and oligonucleiotides ≥ 20 bases long will be retained after repeated hybridization and washing.
References
Gatti RA et al. (1984). Multiple uses of Southern blots. Biotechniques 2, 148-155.
Li JK et al. (1987). Rapid alkaline blot-transfer of viral dsRNAs. Anal Biochem 163, 210-218.
