0.2 ml PCR Tube Strips, Low-Profile

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Overview

PCR Consumables Selection Wizard
PCR Consumables Selection Wizard

Instrument Compatibility
PCR Tube Strip Compatibility
Tube Strip Comparison 0.2 ml PCR Tube Strips, Full-Height 0.2 ml PCR Tube Strips, Low-Profile
Catalog number TBS-xxxx, TBC-xxxx TLS-xxxx
Well height 20.75 mm 15.48 mm
PCR reaction volume 5–125 µl 5–125 µl
Maximum well volume 300 µl 200 µl
Thermal Cycler Compatibility
Bio-Rad C1000/S1000 • •
Bio-Rad DNA Engine, DNA Engine Tetrad and Tetrad 2, DNA Engine Dyad, and Dyad Disciple • •
Bio-Rad iCycler and MyCycler •  
Bio-Rad PTC-100 • •
ABI 0.2ml tube cyclers (2720, 9700, Veriti, etc.) •  
ABI 0.1ml tube cyclers (9800 Fast, Veriti Fast, etc.)   •
Eppendorf Mastercycler series • •
Real-Time PCR Detection System Compatibility
Bio-Rad CFX96   •
Bio-Rad iQ5, iCycler iQ, MyiQ •  
Bio-Rad Chromo4 • •
Bio-Rad DNA Engine Opticon and Opticon 2   •
ABI regular systems (7300, 7500, 7900HT, etc.) •  
ABI fast systems (7500 Fast, 7900HT Fast, StepOne, StepOnePlus, etc.)   •
Eppendorf Mastercycler ep realplex • •
Stratagene Mx series •  
Optical Guide
Optical Guide

Well Color Effect on Signal Strength

Well color effect on signal strength

White well:

  • Increases fluorescent signal for maximizing detection sensitivity
  • Prevents fluorescent interference from adjacent wells or a contaminated cycler

Clear well:

  • Transparent wells for easy sample handling
  • Low signal variability for consistent results

Black well:

  • Lower signal strength for FP (fluorescence polarization) and other assays where reflected light must be minimized

Sealer Effect on Signal Strength

Sealer effect on signal strength

Optical flat cap strips:

  • Vapor-tight sealing for tube strips or 96-well plates
  • Suitable for PCR volumes >5 µl

Microseal® 'B' adhesive seals, optically clear:

  • A convenient sealing film for 96- or 384-well plates
  • Higher optical transmission than optical caps
  • Suitable for PCR volumes >10 µl

Clear Chill-out™ liquid wax:

  • No heated lid or lid pressure is required for PCR
  • Higher optical transmission than optical caps
  • Solidified below 10°C to prevent spilling and aerosol formation
High-Profile vs. Low-Profile
High-Profile vs. Low-Profile

 

There are two common well heights for PCR tubes and plates:

  1. High-profile (full-height; around 20.7 mm) wells are “standard” and fit in most classical thermal cyclers and real-time PCR detection systems, such as Bio-Rad's iCycler®, iQ™ series real-time PCR detection systems, and Applied Biosystems' regular PCR systems and DNA sequencers.
  2. Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR. They have been recommended for newer instruments such as Bio-Rad's C1000 Touch™ and S1000™ thermal cyclers, CFX series real-time PCR detection systems, and Applied Biosystems' fast PCR systems.

Instruments with adjustable lids can usually use both high-profile and low-profile wells. These instruments include Bio-Rad's 1000-series and DNA Engine® series thermal cyclers.

Low-profile 0.2 ml thin-wall polypropylene PCR tube strips are recommended for reaction volumes of 5–125 µl (200 µl maximum). The lower overall height (15.48 mm) of low-profile 0.2 ml tube strips reduces the potential for condensate formation and offers advantages for fast PCR, low-volume reactions, and light capture in fluorescence assays such as real-time PCR. The low-profile V-well tubes can be supported in a 96-place PCR-tube rack and still provide the low clearance needed to fit in some microplate readers. Overall height, including flat optical caps and 96-place rack, is 18.3 mm. Low-profile 0.2 ml tubes can be centrifuged if supported in a 96-place rack, which is also useful for reaction setup and storage.

Low-profile tubes are available in strips of 8 and come in natural and opaque white (white tube strips are useful for optical assays in which increased signal is desired). The tube strips are without caps; use the strip cap tool for quick seating of cap strips on tubes.

Key Features

  • Tight sealing and convenient handling for multiple samples
  • DNase-, RNase-, and human DNA-free

 

Low-Profile 0.2 ml 8-Tube Strips without Caps

TLS-0801
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

List Price:   $95.00
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Low-Profile 0.2 ml 8-Tube Strips without Caps

TLS-0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

List Price:   $99.00
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