Experion RNA StdSens Starter Kit

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Overview

Experion Technology
Experion Technology
Experion System Training
Experion System Training
RNA Integrity Webinar
RNA Integrity Webinar

November 25, 2009
Arnaud Remy and Michael W. Pfaffl

This two-part webinar by Arnaud Remy (Bio-Rad) and Michael W. Pfaffl (Technical University of Munich) discusses the technology and principles of RNA analysis by automated electrophoresis and compares the use of two available platforms for the determinations of RNA integrity, an essential step in quantitative PCR experiments. Duration: 48 min.

Click here to view video
Experion System
Experion System
StdSens Ladder
RNA StdSens Ladder Electropherogram

Electropherogram of the Experion RNA ladder, illustrating relative positions of the lower marker and RNA peaks.

Electropherogram of the Experion RNA Ladder for the mRNA assays illustrating the relative positions of the lower marker and RNA ladder peaks.

Control Total RNA
Control Total RNA Electropherogram

Separation of the 100 ng/µl mouse liver total RNA sample (scaled to local). Results shown are typical for separations of high integrity total RNA from eukaryotic sources. The two most prominent peaks, aside from the lower alignment marker, represent 18S and 28S ribosomal RNA (rRNA).

Pipet Tips
Pipet Tips Recommended for Experion Chip Loading

(Narrow-bore tips such as A and B are recommended. Tips C,D,E not recommended.)


Filter tips are always recommended for RNA work.

Examples of recommended pipet tips for loading chips:

For RNA or DNA work:

  • Rainin L-10F
  • Rainin SR-L10F
  • Rainin GP-L10F
  • VWR 87001-688

For protein work:

  • Rainin GP-L10F
  • Rainin GP-10G
  • Rainin GP-10F
  • Rainin GP-10S
  • Rainin GP-10
  • VWR 87001-688

 

The Experion™ RNA StdSens starter kit is intended for new or experienced users of the Experion automated electrophoresis system. This kit contains all materials necessary to get started with RNA analysis on the Experion system, allowing the user to practice techniques and analyze data or validate the system using a known total RNA standard. Get started or practice with step-by-step instructions and tips for successful microfluidic separations using the provided standard before moving on to your own samples. This kit provides information on:

  • How best to prepare and load an RNA chip
  • How to confirm RNA quality and integrity
  • Tips and common mistakes

The RNA StdSens starter kit contains:

  • Experion StdSens RNA reagents
  • Experion StdSens RNA chips
  • RNase-free polypropylene tips
  • RNase-free polypropylene microcentrifuge tubes
  • DEPC water
  • Spin filters
  • Electrode cleaner
  • Cleaning swabs
  • Cleaning chips
  • Total RNA control sample
  • Detailed instruction manual

The Experion automated electrophoresis system can be used upstream and downstream of a number of other nucleic acid- and protein-application focused products from Bio-Rad.

Components Storage Conditions 
Mouse liver total RNA sample (500 ng/ µl), 20 µl -70°C
DEPC-treated water, 50 ml Room temperature
Experion electrode cleaner, 25 ml Room temperature 
Experion cleaning chips, 2 Room temperature  
Cleaning swabs, 25 Room temperature   
Narrow-bore 20 µl pipet tips, RNase-free, 96 (1 rack) Room temperature    
0.65ml RNase-free microcentrifuge tubes, 50 Room temperature     
Experion RNA StdSens chips, 3 Room temperature      
Reagents for analysis  
RNA gel, 1 vial, 1,250 µl 4°C
RNA loading buffer, 1 vial 900 µl 4°C 
RNA stain, 1 vial, 20 µl 4°C  
Spin filters, 1 4°C  
RNA ladder, 1 vial, 20 µl –70°C   
Experion training video: Chip Loading (DVD) –
Instructions –
Experion RNA StdSens Starter Kit

700-7111
Starter kit for standard-sensitivity RNA analysis, includes 3 RNA StdSens chips, cleaning supplies, reagents, spin filters, RNA standard, tips and tubes, DEPC-treated water

List Price:   $575.00
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RNA Ladder

700-7255
20 μl, RNA size standards, replacement component of Experion RNA analysis kits

List Price:   $46.00
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Experion RNA StdSens Chips

700-7153
Pkg of 10, microfluidic chips for standard-sensitivity RNA analysis with the Experion automated electrophoresis system, comes with 2 cleaning chips

List Price:   $295.00
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Experion Mouse Liver Total RNA Standard

700-7112
20 μl, 500 ng/μl, RNA standard

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Experion Cleaning Chips

700-7251
Pkg of 10, cleaning chip for use with the Experion electrophoresis station (#700-7010)

List Price:   $120.00
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Experion Electrode Cleaner

700-7252
250 ml, for use with Experion cleaning chips (#700-7251)

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Experion DEPC-Treated Water

700-7253
100 ml, DEPC-treated water for use with the Experion automated electrophoresis system, comes with 2 cleaning chips

List Price:   $81.00
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Experion Spin Filters

700-7254
Pkg of 10, spin filter, for use with Experion analysis kits

List Price:   $57.00
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Cleaning Swabs

700-7264
Pkg of 25, swab for use with any Experion analysis kit

List Price:   $38.00
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Number Description Options
BR124 BioRadiations Magazine, Number 124 Click to download [ Add to Cart ]
BR126 BioRadiations Magazine, Number 126 Click to download [ Add to Cart ]
3140 Experion Automated Electrophoresis System Brochure, Rev F Click to download [ Add to Cart ]
3170 Experion RNA HighSens and RNA StdSens Analysis Kits, PIS, Rev E Click to download [ Add to Cart ]
3171 Experion Software PIS, Rev C Click to download [ Add to Cart ]
3174A Experion Folder (includes bulletins 3140, 3169, 3170, 3171, and 3184) [ Add to Cart ]
3184 Experion Video Tour CD [ Add to Cart ]
3186 Experion Automated Electrophoresis System Flier, Rev A Click to download
5253 Experion System Performance Comparison, Rev A Click to download
5285 Bio-Rad Applies Microfluidics to Automate Gel Electrophoresis, Rev C Click to download [ Add to Cart ]
5286 Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for RNA Analysis, Rev A Click to download
5315 Using the Experion Automated Electrophoresis System to Assess RNA Quality and Quantity in siRNA-Induced Gene Silencing Experiments, Rev A Click to download
5420 Experion RNA Quantitation Methods, Rev A Click to download
5452 Effect of RNA Degradation on Data Quality in Quantitative PCR and Microarray Experiments, Rev B Click to download [ Add to Cart ]
5460 Biomarker Discovery Workflow Brochure, Rev B Click to download
5732 Experion Pro260 and RNA StdSens Starter Kits PIS, Rev B Click to download [ Add to Cart ]
5761 Development and Validation of RQI: An RNA Quality Indicator for the Experion Automated Electrophoresis System, Rev B Click to download [ Add to Cart ]
5783 Experion Automated Electrophoresis System Flier, Rev A Click to download [ Add to Cart ]
5859 A Practical Approach to RT-qPCR — Publishing Data That Conform to the MIQE Guidelines, Rev D Click to download [ Add to Cart ]
5868 Performance Comparison of Two LabChip Microfluidic Platforms for Protein, DNA, and RNA Analyses, Rev A Click to download
5893 Myth Busted: A NanoDrop ND-1000 Spectrophotometric Reading Is Insufficient to Assess RNA Quality, Rev A Click to download [ Add to Cart ]
RP0045 Standardization of qPCR and RT-qPCR Click to download
10000976 Instruction Manual, Experion RNA StdSens and HighSens Analysis Kits, Rev C Click to download
10001312 Instruction Manual, Experion Automated Electrophoresis System, Rev C Click to download
10004491 Quick Guide, Experion RNA HighSens Analysis Kit, Rev B Click to download
10004492 Quick Guide, Experion RNA StdSens Analysis Kit, Rev B Click to download
10005250 Instruction Manual, IQ/OQ Protocols and Instuctions, Experion Electrophoresis Station, Rev D Click to download [ Add to Cart ]
10005581 Instruction Manual, Experion Security Edition Software Setup Guide, Rev C Click to download [ Add to Cart ]
10005740 Quick Installation Guide, Experion System Software Windows XP, Rev A Click to download [ Add to Cart ]
10010511 Instruction Manual, Experion RNA StdSens Starter Kit, Rev A Click to download [ Add to Cart ]
10014627 Quick Installation Guide, Experion System Software Windows VISTA, Rev A Click to download