| | 1 x Formulation | Applications |
|
Transfer Buffers*
10 Tris/glycine | 25mM Tris, 192 mM glycine, pH 8.3 | Western Blotting |
| 10x Tris CAPS | Anode buffer; 60 mM Tris,
40 mM CAPS, 15% methanol, pH 9.6 | A discontinuous buffer system that increases ransfer efficiency in semi-dry applications |
| | Cathode buffer, 60 mM Tris,
40 mM CAPS, 0.1% (w/v) SDS, pH 9.6 | |
| 20x SSC | 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0 | Capillary transfer of agarose gels |
Processing Buffers
10x PBS | 10 mM sodium phosphate,
150 mM NaCl, pH 7.4 | Western blotting wash solution |
| 10x TBS | 20 mM Tris,
500 mM NaCl, 1% (w/v) casein, pH 7.4 | Western blotting wash solution |
| 1x PBS with 1% casein | 10 mM sodium phosphate,
150 mM NaCl, 1% (w/v) casein, pH 7.4 | Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes) |
| 1x TBS with 1% casein | 20 mM Tris, 200 mM NaCl, containing 1% (w/v) casein, pH 7.4 | Western blotting blocking buffer (casein blockers recommended for all applications, including those with biotin-avidin complexes) |
| 20x SSC | 150 mM sodium chloride,
15 mM sodium citrate, pH 7.0 | Northern and Southern blotting prehybridization and hybridization solutions |
|
