Detergents and Blocking Reagents for Blotting

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Detergents and Blocking Reagents

1x PBS/1% casein	10 mM sodium phosphate, 150 mM NaCl, 1% (w/v) casein, pH 7.4	Western blotting wash solution (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS/1% casein	20 mM Tris, 500 mM NaCl, 1% (w/v) casein, pH 7.4	Western blotting wash solution (casein blockers recommended for all applications, including those with biotin-avidin complexes)
10% Tween 20	10% (w/v) Tween 20	Blocking and wash buffer component

The processing of blots is now even simpler with a variety of premixed wash buffers and blocking solutions:

Premixed blocking buffers, available as PBS/casein and TBS/casein, take the time and effort out of solubilizing casein
10% Tween 20 makes pipetting of Tween 20 accurate and simple

170-6537
200 g, enzyme immunoassay grade gelatin

170-6404
300 g, nonfat dry milk for western blotting applications

170-6531
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)

161-0781
1 L, detergent, for easy pipetting

161-0418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution

161-0783
1 L, blocking reagent

161-0782
1 L, blocking reagent

Number	Description	Options
2317	Ready-to-Run Buffers and Solutions Brochure, Rev D	Click to download	[ Add to Cart ] Check Out

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