Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than on their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.
Ready Gel® precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by electrophoresis on Ready Gel precast gels using the Mini-PROTEAN® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN® 3 Dodeca™ cell (1–12 gels).