Ready Gel IEF Precast Gels

print email

Overview

Migration Pattern for Ready Gel IEF Gels

The banding patterns indicate the optimal separation by isoelectric point (pI) for each acrylamide percentage in combination with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Mini Format Precast Gel Selection Guide

Mini-Format Precast Gel Selection Guide

Refer to the following table to select the appropriate gel type and buffers for your polyacrylamide gel-based applications.


Gel Type	Application	Sample Buffer	Running Buffer
Tris/Glycine	SDS-PAGE Native PAGE	Laemmli Native	Tris/glycine/SDS Tris/glycine
Tris-Tricine	SDS-PAGE	Laemmli	Tris/Tricine/SDS
IEF	IEF	IEF	Anode and cathode buffer
Zymogram	Protease detection	Zymogram	Tris/glycine/SDS

Isoelectric focusing (IEF) gels separate proteins based on their net charge rather than on their molecular weight. IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.

Ready Gel^® precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by electrophoresis on Ready Gel precast gels using the Mini-PROTEAN^® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN^® 3 Dodeca™ cell (1–12 gels).

Gel dimensions (W x L x thickness)	8.6 x 6.8 x 0.1 cm
Cassette dimensions (W x L x thickness)	10 x 8 x 0.4 cm
Gel storage conditions	Store flat at 4°C; do not freeze
Gel shelf life*	~26 weeks
* From date of manufacture.