Bio-Plex® Nonmagnetic Signal Transduction Assays

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Overview

Available Nonmagnetic Assays

Assays Phosphoprotein Total Bead Region
Akt Signaling
Akt (Ser473) 75
GSK-3α/β (Ser21/Ser9)  — 18
Cell Cycle/Checkpoint Control
p53 (Ser15) 53
p53 (Ser37) 53
p53 (Ser46) 53
Chromatin Regulation/Acetylation
Histone H3 (Ser10)  — 77
Immunology/Inflammation
IκB-α (Ser32/Ser36) 58
NF-κB p65 (Ser536)  — 37
STAT2 (Tyr689)  — 32
STAT3 (Ser727)  — 52
STAT3 (Tyr706)  — 52
STAT6 (Tyr641)  — 72
Tyk2 (Tyr1054/Tyr1055)  — 33
Glucose/Energy Metabolism
IGF-IR (Tyr1131)  — 43
IR-β (Tyr1146) 43
IRS-1 (Ser636/Ser639)  — 76
MAP Kinase Signaling
ATF-2 (Thr71) 20
c-Jun (Ser63) 56
ERK1 (Thr202/Tyr204)  — 38
ERK2 (Thr185/Tyr187) 38
ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) 38
HSP27 (Ser78) 51
JNK (Thr183/Tyr185) 34
MEK1 (Ser217/Ser221) 40
p38 MAPK (Thr180/Tyr182) 36
p90RSK (Thr359/Ser363) 35
Neuroscience
CREB (Ser133) 19
TrkA (Tyr490)  — 73
Translational Control
p70 S6 kinase (Thr421/Ser424)  — 55
S6 ribosomal protein (Ser236/Ser236)  — 74
Tyrosine Kinases
Bcr-AbI (Tyr245) 45
c-AbI (Tyr245)
45
c-AbI (Tyr412)  — 45
EGFR (Tyr)  — 20
PDGF receptor-β (Tyr751)  — 57
Src (Tyr416)  — 41

 

CST antibodies exclusively developed and validated for Bio-Plex phosphoprotein and total target assays.


Multiplexability Matrix

Assay multiplexability matrix shows representative data based on lysates provided with the assay.

To read more click here

What You Need For Bio-Plex Signal Transduction Assays
Bio-Plex phosphoprotein and total target assays and associated reagents are designed to provide up to 96 data points for each assay. Two packaging options are available: one 96-well plate and ten 96-well plates. For phosphoprotein detection, you will need to order the following components:

Bio-Plex phosphoprotein assays and Bio-Plex total target assays — includes coupled beads, detection antibodies, and lysates to test for specific proteins of interest in lysate samples.

Bio-Plex cell lysis kit — includes cell lysis buffer, cell wash buffer, factor 1 and factor 2; essential for optimal lysis of your cell culture or tissue samples.

Bio-Plex phosphoprotein detection reagent kit — includes assay buffer, wash buffer, antibody diluent, resuspension buffer, streptavidin-PE, 96-well filter plate, sealing tape, and instructions required to prepare assays and acquire data.

Bio-Plex Assay Builder

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder



Bio-Plex® phosphoprotein assays are bead-based multiplexable assays that directly detect phosphorylated proteins in lysates derived from cell culture or tissue samples. Bio-Plex total target assays measure the total abundance of a target protein, regardless of its phosphorylation state, and can be used to report the total concentration of a protein. Bio-Plex phosphoprotein assays measure the concentration of the phosphorylated protein, only. In cases where phosphoprotein and total protein assays are offered, they must be run in different wells due to shared bead regions. Using the multiplex format, the profiles of multiple phosphorylated proteins involved in cell signaling pathways can be detected using only 25 µl of lysate.

These assays are based on 5.6 µm nonmagnetic beads. For the multiplexing capabilities of specific assay combinations, see bulletin 5483.

Key Features

  • Highly specific antibodies exclusively developed and validated by Cell Signaling Technology, Inc.
  • 96-well filter-plate format which yields significantly higher throughput than traditional methods
  • Higher specificity sandwich format which minimizes cross reactivity with other cellular proteins
  • Direct measurement of kinases that have been activated within the cell
  • Low sample volume requirement
  • Minimal hands-on times

Additional Neccessary Reagents

In addition to a phosphoprotein or total target assay, the following items are needed to perform a Bio-Plex signal transduction assay:

  • Bio-Plex phosphoprotein detection reagent kit (#171-304004, 1 x 96-well) — includes assay reagents and instructions required to prepare samples and acquire data
  • Bio-Plex cell lysis kit (#171-304011, 1 x 96-well) — includes buffers and factors essential for the optimal lysis of cell culture or tissue samples

Other Assay Formats Available

Instrument Compatibility

  • Bio-Plex 200 system
  • Bio-Plex 3D system
  • Bio-Plex Pro™ II wash station
Reactive species Human, mouse, rat, monkey
Matrices Cell culture lysate, tissue lysate
Sample volume, µl 25
Limit of detection, µl/ml of total protein <10
   Intra-assay CV
   Inter-assay CV
<15%
<25%
Multiplexing capability Yes
Storage condition 2–8°C for coupled beads and detection antibodies
–20°C for lysates (shipped separately)
Stability* 12 months

Values vary by assay.
* Check label for expiration date.

 
Immunoassay for Signal transduction
Species Human, Mouse, Rat, Monkey
Custom assay options x-Plex™ only
Bio-Plex Phospho-Akt (Ser473) Assay #171-V21075
Bio-Plex Phospho-Akt (Ser473) Assay

171-V21075
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated Akt (Ser473), requires reagent kit and cell lysis kit

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Bio-Plex Total Akt Assay #171-V31075
Bio-Plex Total Akt Assay

171-V31075
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total Akt, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-ATF-2 (Thr71) Assay #171-V21620
Bio-Plex Phospho-ATF-2 (Thr71) Assay

171-V21620
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated ATF-2 (Thr71), requires reagent kit and cell lysis kit

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Bio-Plex Total ATF-2 Assay #171-V31620
Bio-Plex Total ATF-2 Assay

171-V31620
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total ATF-2, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-Bcr-Abl (Tyr245) Assay #171-V27145
Bio-Plex Phospho-Bcr-Abl (Tyr245) Assay

171-V27145
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated Bcr-Abl (Tyr245), requires reagent kit and cell lysis kit

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Bio-Plex Total Bcr-Abl Assay #171-V37145
Bio-Plex Total Bcr-Abl Assay

171-V37145
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total Bcr-Abl, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-c-Abl (Tyr245) Assay #171-V27545
Bio-Plex Phospho-c-Abl (Tyr245) Assay

171-V27545
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated c-Abl (Tyr245), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-c-Abl (Tyr412) Assay #171-V27745
Bio-Plex Phospho-c-Abl (Tyr412) Assay

171-V27745
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated c-Abl (Tyr412), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-c-Jun (Ser63) Assay #171-V24356
Bio-Plex Phospho-c-Jun (Ser63) Assay

171-V24356
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated c-Jun (Ser63), requires reagent kit and cell lysis kit

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Bio-Plex Total c-Jun Assay #171-V34356
Bio-Plex Total c-Jun Assay

171-V34356
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total c-Jun, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-CREB (Ser133) Assay #171-V26119
Bio-Plex Phospho-CREB (Ser133) Assay

171-V26119
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated CREB (Ser133), requires reagent kit and cell lysis kit

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Bio-Plex Total CREB Assay #171-V36119
Bio-Plex Total CREB Assay

171-V36119
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total CREB, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-EGFR (Tyr) Assay #171-V23120
Bio-Plex Phospho-EGFR (Tyr) Assay

171-V23120
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated EGFR (Tyr), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-ERK1 (Thr202/Tyr204) Assay #171-V21938
Bio-Plex Phospho-ERK1 (Thr202/Tyr204) Assay

171-V21938
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated ERK1 (Thr202/Tyr204), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-ERK2 (Thr185/Tyr187) Assay #171-V20438
Bio-Plex Phospho-ERK2 (Thr185/Tyr187) Assay

171-V20438
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated ERK2 (Thr185/Tyr187), requires reagent kit and cell lysis kit

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Bio-Plex Total ERK2 Assay #171-V30438
Bio-Plex Total ERK2 Assay

171-V30438
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total ERK2, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) Assay #171-V22238
Bio-Plex Phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187) Assay

171-V22238
1 x 96-well, includes coupled beads, detection antibodies, and lysates; detects phosphorylated ERK1/2 (Thr202/Tyr204, Thr185/Tyr187); requires reagent kit and cell lysis kit

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Bio-Plex Total ERK1/2 Assay #171-V32238
Bio-Plex Total ERK1/2 Assay

171-V32238
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total ERK1/2, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-Histone H3 (Ser10) Assay #171-V24777
Bio-Plex Phospho-Histone H3 (Ser10) Assay

171-V24777
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated Histone H3 (Ser10), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-HSP27 (Ser78) Assay #171-V24551
Bio-Plex Phospho-HSP27 (Ser78) Assay

171-V24551
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated HSP27 (Ser78), requires reagent kit and cell lysis kit

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Bio-Plex Total HSP27 Assay #171-V34551
Bio-Plex Total HSP27 Assay

171-V34551
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total HSP27, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-IGF-IR (Tyr1131) Assay #171-V27343
Bio-Plex Phospho-IGF-IR (Tyr1131) Assay

171-V27343
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated IGF-IR (Tyr1131), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-I?B-a (Ser32/Ser36) Assay #171-V20758
Bio-Plex Phospho-IκB-α (Ser32/Ser36) Assay

171-V20758
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated IκB-α (Ser32/Ser36), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-IR-ß(Tyr1146) Assay #171-V27943
Bio-Plex Phospho-IR-β(Tyr1146) Assay

171-V27943
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated IR-β (Tyr1146), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-IRS-1 (Ser636/Ser639) Assay #171-V25576
Bio-Plex Phospho-IRS-1 (Ser636/Ser639) Assay

171-V25576
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated IRS-1 (Ser636/Ser639), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-JNK (Thr183/Tyr185) Assay #171-V21034
Bio-Plex Phospho-JNK (Thr183/Tyr185) Assay

171-V21034
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated JNK (Thr183/Tyr185), requires reagent kit and cell lysis kit

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Bio-Plex Total JNK Assay

171-V31034
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total JNK, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-MEK1 (Ser217/Ser221) Assay

171-V25340
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated MEK1 (Ser217/Ser221), requires reagent kit and cell lysis kit

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Bio-Plex Total MEK1 Assay

171-V35340
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total MEK1, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-NF-κB p65 (Ser536) Assay

171-V24937
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated NF-κB p65 (Ser536), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-p38 MAPK (Thr180/Tyr182) Assay

171-V21336
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated p38 MAPK (Thr180/Tyr182), requires reagent kit and cell lysis kit

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Bio-Plex Total p38 MAPK Assay

171-V31336
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total p38 MAPK, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-p53 (Ser15) Assay

171-V25153
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated p53 (Ser15), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-p53 (Ser37) Assay

171-V28353
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated p53 (Ser37), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-p53 (Ser46) Assay

171-V28153
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated p53 (Ser46), requires reagent kit and cell lysis kit

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Bio-Plex Total p53 Assay

171-V35153
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total p53, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-p70 S6 Kinase (Thr421/Ser424) Assay

171-V24155
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated p70 S6 Kinase (Thr421/Ser 424), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-p90RSK (Thr359/Ser363) Assay

171-V23535
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated p90RSK (Thr359/Ser363), requires reagent kit and cell lysis kit

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Bio-Plex Total p90RSK Assay

171-V33535
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total p90RSK, requires reagent kit and cell lysis kit

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Bio-Plex Phospho-PDGF Receptor-β (Tyr751) Assay

171-V25957
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated PDGF Receptor-β (Tyr751), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-Src (Tyr416) Assay

171-V26741
1 x 96-well, includes coupled beads, detection antibodies, and lysates for detecting phosphorylated Src (Tyr416), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-S6 Ribosomal Protein (Ser235/Ser236) Assay

171-V25374
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated S6 Ribosomal Protein (Ser235/Ser236), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-STAT2 (Tyr689) Assay

171-V23732
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated STAT2 (Tyr689), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-STAT3 (Ser727) Assay

171-V26952
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated STAT3 (Ser727), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-STAT3 (Tyr705) Assay

171-V22552
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated STAT3 (Tyr705), requires reagent kit and cell lysis kit

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Bio-Plex Phospho STAT6 (Tyr641) Assay

171-V25772
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated STAT6 (Tyr641), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-TrkA (Tyr490) Assay

171-V23973
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated TrkA (Tyr490), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-GSK-3α/β (Ser21/Ser9) Assay

171-V23318
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated GSK-3α/β (Ser21/Ser9), requires reagent kit and cell lysis kit

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Bio-Plex Phospho-Tyk2 (Tyr1054/Tyr1055) Assay 1 x 96-well

171-V26533
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting phosphorylated Tyk2 (Tyr1054/Tyr1055), requires reagent kit and cell lysis kit

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Bio-Plex Total IκB-α Assay

171-V30758
1 x 96-well, includes coupled beads, detection antibodies, and lysates, for detecting total IκB-α, requires reagent kit and cell lysis kit

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Number Description Options
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart ]
6335 Bio-Plex Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev B Click to download
3033 Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A Click to download
4110186 Bio-Plex Phosphoprotein Assay Lysate Table, Rev D Click to download
3034 Bio-Plex Phosphoprotein Assays Feature Cell Signaling Technology (CST) Antibodies Product Information Sheet, Rev C Click to download [ Add to Cart ]
2903 Bio-Plex Phosphoprotein Detection Brochure, Rev F Click to download
5483 Bio-Plex Phosphoprotein Detection Performance Data Sheet, Rev E Click to download [ Add to Cart ]
4110019 Bio-Plex Phosphoprotein Detection Product Insert, Rev F Click to download
5394 Bio-Plex Phosphoprotein Detection References, Rev B Click to download
5507 Bio-Plex Multiplex System Ordering Information Sheet, Interactive PDF, Rev K Click to download
5590 Detection of Genetic Abnormalities in Cancer Cells Using Bio-Plex Immunoassays, Rev A Click to download
3141 Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A Click to download
5579 Downstream EGFR Protein Phosphorylation Tech note, Rev A Click to download
5312 Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays, Rev A Click to download
4110051 Instruction Manual, Bio-Plex Cell Lysis Kit Product Insert, Rev D Click to download [ Add to Cart ]
4110018 Instruction Manual, Bio-Plex Phosphoprotein Testing, Rev C Click to download [ Add to Cart ]
5377 Lysate Preparation for Bio-Plex Phospho-Histone H3 Assay, Rev A Click to download
5482 The Power of xMAP Technology (xMAP vs. WB Comparison) Flier, Rev C Click to download [ Add to Cart ]
2927 Quick Guide, Bio-Plex Phosphoprotein Testing, Rev B Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
2981 Simultaneous Detection of 12 Phosphorylated and 7 Total Proteins Using the Bio-Plex Suspension Array System, Rev B Click to download
5296 Simultaneous Detection of Multiple Phosphoprotein Targets From Human Tumor Tissues Using the Bio-Plex Suspension Array System, Rev A Click to download
5301 Target Expression and Target Modulation Studies in Patient-Derived Tumor Xenografts Using a Bank of Protein Lysates, Rev A Click to download
5863 Using Bio-Plex Phosphoprotein Assays to Study EGFR Signaling in Human Patient-Derived Xenografts Treated with Cetuximab, Rev A Click to download