PCR Kleen Spin Columns

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Overview

PCR/DNA Purification Guide
PCR Product and DNA Fragment Purification Guide

Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.

  Freeze 'N Squeeze DNA
Gel Extraction Spin Columns		 Micro Bio-Spin and
Bio-Spin Columns		 PCR Kleen
Spin Columns
Application Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting Removal of PCR products >200 bp from other reaction mixture components
Starting Material ≤700 mg gel slice 10–100 µl of sequencing reaction mixture 25–100 µl of PCR or other enzyme reaction
Format Spin-column extraction of frozen-thawed gel slices Prepacked size exclusion spin column Prepacked size exclusion spin column
Recovery 4–23 kb: >80% recovery;
0.5–4 kb: >70% recovery;
50–500 bp: >50% recovery		 >95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators 50–80%
Time Required <10 min <10 min <4 min
Rapid Removal of Primer-Dimers
Rapid Removal of Primer-Dimers

Effective, rapid removal of primer-dimers from PCR reactions. Two PCR reaction mixtures were purified with PCR Kleen spin columns. Lane 1, a mixture of 2 µg of a 660 bp DNA fragment with 100 ng of a 25-mer primer-dimer; lane 3, a mixture of 2 µg of a 660 bp DNA fragment with 500 ng of a 25-mer primer; lane 2, primer-dimer purification; lane 4, primer purification; M, DNA markers. Reaction products and purified samples (10 µl each) were analyzed on a 1.5% agarose gel.

PCR Kleen columns are prepacked spin columns for purifying PCR products and other DNA molecules >200 bp directly from reaction mixtures. A simple 4 minute spin effectively removes salts, nucleotides, enzymes, primers, and primer-dimers. Purified DNA fragments are eluted into a collection tube and are immediately available for secondary PCR, subcloning, restriction digests, ligations, sequencing, and other enzymatic manipulations.

Method Size exclusion chromatography in spin column
Applications Removal of nucleic acids <32 bases and other reaction mixture components from PCR products >200 bp
Capacity 25–100 µl
Equllibration buffer 10 mm Tris, 1mM EDTA, pH 7.4
Sample preparation time <4 min
Yield 50–80% recovery
PCR Kleen Spin Columns

732-6300
Pkg of 25, prepacked spin column, for purification of PCR products and other DNA fragments >200 bp directly from reaction mixtures

List Price:   $78.75
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Number Description Options
2950 Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A Click to download
2311 PCR Kleen Spin Columns Product Information Sheet, Rev D Click to download
4006142 Instruction Manual, Quantum Prep PCR Kleen Spin Columns, Biotechnology Explorer, Rev B Click to download