Certified Genetic Quality Tested (GQT) DNA Grade Agarose

All Bio-Rad Certified agarose products are guaranteed to be free of inhibitors, DNases, and RNases.

Certified molecular biology agarose — This is a linear polymer with a very high molecular weight, which results in a gel structure unlike those of traditional agaroses. This general-purpose agarose ensures that DNA recovered from a preparative gel can be manipulated without compromising quality. With a very low sulfate content, this general-purpose agarose produces a gel with a very high gel strength and higher exclusion limit. The high electrophoretic mobility increases resolution and reduces run time, and the gels are easy to handle even at low agarose percentages. Analytical separation is ≥1,000 bp.

Certified PCR agarose — Certified PCR agarose is recommended for separation of DNA fragments ≤1,000 bp. This high-strength agarose forms gels that are easy to handle and that remain flexible even at high gel percentages, which minimizes the risk of cracking or breaking. PCR agarose, unlike GQT products with similar sieving properties that gel at higher temperatures, gels at 40°C so it is faster and easier to prepare.

Certified low range ultra agarose — This agarose provides superior resolution of small PCR fragments and primers at lower concentrations than standard agaroses. A 3% gel clearly resolves a 10 bp ladder, and a 4% gel approaches the resolution of an 8% polyacrylamide gel. Analytical separation is 20–800 bp.

Certified megabase agarose — With a very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle at concentrations as low as 0.3% and allow shorter run times. The separation range is between 1 kb and 5 Mb. Low background staining also provides superior imaging of high molecular weight DNA.

Certified low-melt agarose — This low-melting-temperature agarose has a high resolving capacity for DNA fragments ≥1,000 kb. It is recommended for preparative electrophoresis and for in-gel applications such as digestion, ligation, PCR, transformation, and sequencing. It is also recommended for embedding chromosomes and megabase-sized DNA for pulsed field applications.

Certified PCR low-melt agarose — This agarose has a high sieving capacity and yields excellent resolution of fragments ≤1,000 bp in a low-melt or preparative format. This agarose is ideal for digestion by agarose and for all in-gel applications.

Pulsed field Certified agarose — This agarose enables excellent separation and resolution of large DNA fragments in pulsed field gel applications. The optimal separation range is 1 kb–2 Mb. Although gels cast with this agarose have longer PFGE run times than Certified megabase agarose, running conditions for this agarose are a preset selectable method of the CHEF Mapper XA system auto-algorithm.