Chill-out liquid wax provides an excellent vapor barrier that may be used instead of mineral oil in thermal cyclers without heated lids. After cycling, the tubes are chilled below 10°C to harden the wax. The solid layer protects samples from spills or aerosol formation, but is easy to penetrate with a pipet tip for sample retrieval. Chill-out liquid wax is available in a clear formulation (for use in fluorescence assays such as real-time PCR) and a bright red formulation (easily visible when recovering reaction products).
Note: Chill-out wax is not recommended for sealing Microseal 384-well PCR plates.
