Ready Gel Zymogram Precast Gels

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Overview

Migration Pattern
Migration Pattern for Ready Gel Zymogram Gels

The banding patterns indicate the optimal separation ranges (in kD) for each acrylamide percentage in combinations with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Mini Format Precast Gel Selection Guide
Mini-Format Precast Gel Selection Guide
Refer to the following table to select the appropriate gel type and buffers for your polyacrylamide gel-based applications.
 
Gel Type


Application



Sample Buffer Running Buffer
Tris/Glycine


SDS-PAGE
Native PAGE



Laemmli
Native		 Tris/glycine/SDS
Tris/glycine
Tris-Tricine


SDS-PAGE



Laemmli Tris/Tricine/SDS
IEF


IEF



IEF Anode and cathode buffer
Zymogram


Protease detection



Zymogram Tris/glycine/SDS


Zymogram gels contain either gelatin or casein, which are substrates for various proteases. Samples are run under denaturing but nonreducing conditions, then allowed to renature and consume the substrate. Samples with proteolytic activity can be visualized as clear bands against a blue background after Coomassie Blue R-250 staining.

Ready Gel® precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by electrophoresis on Ready Gel precast gels using the Mini-PROTEAN® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN® 3 Dodeca™ cell (1–12 gels).

Gel dimensions (W x L x thickness) 8.6 x 6.8 x 0.1 cm
Cassette dimensions (W x L x thickness) 10 x 8 x 0.4 cm
Gel storage conditions Store flat at 4°C; do not freeze
Gel shelf life* 8–12 weeks for Tris-HCI, Tris-Tricine, zymogram, TBE, TBE-urea
* From date of manufacture.  
Ready Gel Zymogram Gel

161-1113
Pkg of 1, 10% precast polyacrylamide gel with gelatin, 10-well, 30 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Ready Gel Zymogram Gel

161-1185
Pkg of 1, 10% precast polyacrylamide gel with gelatin, 12-well, 20 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Ready Gel Zymogram Gel

161-1131
Pkg of 1, 10% precast polyacrylamide gel with gelatin, 15-well, 15 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Ready Gel Zymogram Gel

161-1167
Pkg of 1, 10% precast polyacrylamide gel with gelatin, 10-well, 50 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Ready Gel Zymogram Gel

161-1114
Pkg of 1, 12% precast polyacrylamide gel with casein, 10-well, 30 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Ready Gel Zymogram Gel

161-1168
Pkg of 1, 12% precast polyacrylamide gel with casein, 10-well, 50 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Number Description Options
5535 Mini-PROTEAN Electrophoresis System Brochure, Rev E Click to download [ Add to Cart ]
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel System Resource Guide (Note: large PDF file - 3.8 MB) Click to download [ Add to Cart ]
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download
4106111 Instruction Manual, Zymogram Sample Buffer, Rev B Click to download