PROTEAN^® II xi Cell

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Overview

	PROTEAN II® xi (16 cm)	PROTEAN II xi (20 cm)	PROTEAN II XL (20 cm)
Number of gels	1–4	1–4	1–4
Gel size (W x L)	16 x 16 cm (handcast)	16 x 20 cm (handcast)	18.3 x 20 cm (handcast) 18.3 x 19.3 cm (precast)
Glass plate size (W x L)
Inner plate	20 x 16 cm	20 x 20 cm	20 x 20 cm
Outer plate	20 x 18.3 cm	20 x 22.3 cm	20 x 22.3 cm
Spacer length	18.3 cm	22.3 cm	22.3 cm
Typical upper buffer volume	350 ml	350 ml	350 ml
Typical lower buffer volume	1.8 L	1.2 L	1.2 L
Typical run times for SDS-PAGE
Without cooling	4 hr	5 hr	5 hr
With cooling	2.5 hr	3.5 hr	3.5 hr
Recommended power supply	PowerPac™ HV or PowerPac Universal	PowerPac™ HV or PowerPac Universal	PowerPac™ HV or PowerPac Universal

Component Comparison

PROTEAN^® II xi and XL (IPG) component comparison

The 4 mm xi and 13 mm XL clamp notch
The 19 mm xi and 8 mm XL spacer
The 181 mm xi and 198 mm XL central cooling core gasket
The 153 mm xi and 184 mm XL comb

Sample Volume Table

Sample Volume per Well for PROTEAN^® II xi and XL Combs*

		Comb Thickness
Number or Type of Wells	Well Width	0.5 mm	0.75 mm	1.0 mm	1.5 mm	3.0 mm
25	3.5 mm	–	60 µl	80 µl	120 µl	–
20	5.0 mm	54 µl	82 µl	110 µl	164 µl	328 µl
15	6.5 mm	74 µl	110 µl	147 µl	221 µl	442 µl
10	1.0 cm	114 µl	172 µl	229 µl	343 µl	687 µl
5	2.3 cm	–	–	522 µl	783 µl	1.57 ml
3	4.0 cm	–	–	–	1.37 ml	–
Blank	14.5 cm	–	2.44 ml	3.26 ml	4.88 ml	9.76 ml
2-D Comb
Reference well	3.5 mm	–	–	28 µl	42 µl	84 µl
Sample well	17.8–17.9 cm	–	–	–	–	–
* At standard 25 mm well depth.

The PROTEAN^® II xi cell is a large format vertical electrophoresis cell that is used for the most common electrophoretic techniques such as SDS-PAGE, native electrophoresis, and agarose gel electrophoresis. The PROTEAN II xi cell is available in two sizes to accommodate up to four* 16 x 16 cm or 16 x 20 cm gels.

Key Features

Accommodates handcast gels
Patented single-screw clamps on the casting stand that exert uniform pressure along the entire length of the glass plates provide a leakproof seal without agarose plugs or grease
Central cooling core can be connected to tap water or a cooled recirculating bath, or filled with coolant, providing smile-free patterns with as little as 1.5 L of buffer
Choice of glass plates, spacers, and sandwich clamps to cast 2 gel lengths, 16 or 20 cm
Ability to cast multiple gradient gels using the PROTEAN II multi-gel casting chamber, the Model 495 gradient former, and the appropriate exponential piston. Use of the gel clip facilitates handling of large gels
Notched plates allow up to four 16 x 16 cm or 16 x 20 cm handcast gels to be run simultaneously (not recommended for 2-D applications)
Optional frosted glass plates allow high-resolution vertical agarose electrophoresis of nucleic acids
Comb conversion screws convert any PROTEAN II xi comb from a standard 25 mm well depth to a 10 mm depth to simplify comb removal

PROTEAN II IPG Conversion Kits for 2-D Applications

Conversion kits expand the capabilities of the PROTEAN II xi cell to include applications such as tube gel IEF and second-dimension SDS-PAGE. To convert the PROTEAN II xi cell into a:

PROTEAN xi 2-D cell — use available adaptors and other accessories to accommodate 16 tube gels for IEF applications
PROTEAN II XL cell — use the PROTEAN II IPG conversion kit for second-dimension electrophoresis of 17 cm IPG strips

Each cell includes the central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, 4 sandwich clamps, upper buffer dam, casting stand with gaskets, leveling bubble, and instructions. The 16 cm cells include clamps for running 16 cm gels; the 20 cm cells include clamps for 20 cm gels. Cells without combs and spacers (catalog #165-1801 and #165-1811) allow you to select the spacer thickness and comb that best fit your application. See the Accessories tab for a list of available combs and spacers.

* For higher throughput, the PROTEAN II xi multi-cell provides 6-gel capacity for second-dimension runs.