Innovative Optical Design
The CFX384 Touch™ system's solid-state optical technology (five filtered LEDs and five filtered photodiodes) provides sensitive detection for precise quantification and target discrimination.
Scanning just above the sample plate, the optics shuttle individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk. The optical system automatically collects data from all wells during data acquisition, so you can enter or edit well information on your own schedule.
Four-Target Multiplexing
The CFX384 Touch system can discriminate up to four targets in a single reaction well. The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always fixed and optimal, and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference.
Fast Scan Option
You can complete SYBR® Green I and single-color FAM protocols even faster using the fast scan mode, which reads single-channel fluorescence from all 384 wells in just 8 seconds.
Fluorescence Resonance Energy Transfer (FRET)
The CFX384 Touch system's optics shuttle includes one channel with an LED-filter photodiode combination dedicated for FRET singleplex experiments, further expanding your experimental options.
As the CFX384 Touch optics shuttle travels across the plate, light is focused directly into the center of each sample well. Side view of the optics shuttle shows the green LED firing over a well.
Five-Channel Optics Shuttle of the CFX384 Touch System
CFX384 Touch Excitation and Detection Wavelengths
Excitation and detection wavelength ranges for the CFX384 Touch system.
