Analysis of Precut Lambda DNA Kit

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Overview

Purchasing Guide Table
Purchasing Guide Table

Reduce. Reuse. Refresh.

Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase.


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Lab Preparation Checklist Lab Preparation Checklist
Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
PstI lambda DNA digest 1
EcoRI lambda DNA digest 1
Lambda DNA, uncut 1
DNA size standard (HindIII lambda DNA digest) 1
Sample loading buffer, 5x, 1 ml 1
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 60
Foam floats 8
Gel staining trays 4
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Horizontal gel electorphoresis chambers 4–8
Adjustable micropipets
    2–20 µl 4–8
    20–200 µl 1
Pipet tips, 2–200 µl, BR-35 1 bag
Power supplies 2–4
Recommended (Optional) Accessories:
Rocking platform  
Gel support film  
Microwave oven  
DNA Flowchart DNA Flowchart
Analysis of Precut Lambda DNA Flowchart
Analysis of Precut Lambda DNA Flowchart
Key Kit Features Key Kit Features
Key Kit Features
  • Aligns with AP Biology Lab 6
  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Construct standard curves from student data
  • Make precise determinations of DNA fragment sizes
  • Complete in two 45 minute lab sessions
Curriculum Fit Curriculum Fit
Analysis of Precut Lambda DNA Kit Curriculum Fit
Analysis of Precut Lambda DNA Kit Curriculum Fit
Lambda Bacteriophage Lambda Bacteriophage
Lambda Bacteriophage
Lambda Bacteriophage DNA Gel

Lambda bacteriophage has been a workhorse of molecular biology for decades. Itis vital in the fields of molecular cloning and genomic sequencing since it can be used to subclone very long genomic DNA fragments much more efficiently than plasmid vectors. Lambda DNA comes from a bacterial virus, or bacteriophage, which attacks bacteria by injecting them with its nucleic acid. Once inside, Lambda DNA hijacks the bacterial cellular machinery and replicates itself until the cells burst, releasing millions more bacteriophage to carry out the same infection process. Bacteriophage lambda is harmless to humans and other eukaroytic organisms, and therefore makes an excellent source of DNA for experimental study.

Large Class Preparation Guide
Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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Complete Purchasing Guide
Complete Purchasing Guide

Download the complete Biotechnology Explorer™ Refresh Kit Components Purchasing Guide.


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 Bulletin 5872

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. The Analysis of Precut Lambda DNA Kit demonstrates basic procedures and principles of DNA gel electrophoresis, including agarose gel casting, sample loading, size-based separation of DNA fragments, DNA staining, and graphic analysis. This activity provides in-depth explanations about how restriction enzymes cut DNA and how electrophoresis can be used to separate and visualize DNA fragments.

Features and Benefits

  • Aligns with AP Biology Lab 6

With this kit, students are able to:

  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Construct standard curves from their data and make precise determinations of DNA fragment sizes

Applications and Uses

By visualizing the effect of three restriction enzymes on four identical samples of double-stranded lambda virus DNA, students learn that different restriction enzymes recognize and cut different DNA sequences. Optional extension exercises guide students through the procedure of DNA fragment size determination by constructing a standard curve using their own gel data. Students can then use the standard curve to determine the sizes of the various DNA fragments in their samples.

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
PstI lambda DNA digest 1
EcoRI lambda DNA digest 1
Lambda DNA, uncut 1
DNA size standard (HindIII lambda DNA digest) 1
Sample loading buffer, 5x, 1 ml 1
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 60
Foam floats 8
Gel staining trays 4
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Analysis of Precut Lambda DNA Kit

166-0001EDU
DNA gel electrophoresis classroom study kit, includes DNA standard and digests, buffers, agarose, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students; education use only

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Number Description Options
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download
3054 Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Quick Guide Please login to download this document
4006101 Instruction Manual, Analysis of Precut Lambda DNA Kit, Biotechnology Explorer, Rev E Please login to download this document [ Add to Cart ]
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide, Rev A Click to download
5872 Biotechnology Explorer Refresh Kit Components Purchasing Guide, Rev A Click to download
5914 DNA Staining Using Fast Blast DNA Stain or SYBR Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software, Rev A Click to download