Sequi-Gen GT System

print email

Overview

IPC Dimensions	Gel Area	Minimum Buffer Volume		Recommended Constant Power*	Expected Voltage*	Expected Current*	Expected Temperature*
IPC Dimensions	Gel Area	Upper	Lower	Recommended Constant Power*	Expected Voltage*	Expected Current*	Expected Temperature*
21 x 40 cm	17 x 40 cm	500 ml	350 ml	40 W	1,600 V	25 mA	50°C
21 x 50 cm	17 x 50 cm	550 ml	350 ml	50 W	2,000 V	25 mA	50°C
38 x 30 cm	34 .5 x 30 cm	700 ml	350 ml	70 W	2,200 V	32 mA	50°C
38 x 50 cm	34 .5 x 50 cm	1,400 ml	350 ml	80 W	2,500 V	32 mA	50°C

* Typical electrophoresis conditions for a 0.4 mm, 6% acrylamide gel in 1x TBE buffer.

Simple Horizontal Gel Casting Method

Assembling and casting with the Sequi-Gen GT cell is convenient and reliable.

The lever clamps slide over the gel sandwich.
Each clamp exerts an even pressure over the entire length of the glass plate sandwich, resulting in a positive seal. This prevents damage to the plates or leakage, which can result from uneven pressure. Tape, screws, and binder clamps are unnecessary.
With the new casting method, a precision caster is attached to the bottom of the Sequi-Gen GT cell. This assembly is placed flat on the lab bench, and the gel solution is injected with a syringe through an injection port in the precision caster.

Simple as that!

Interchangeable Sequi-Gen GT IPC sizes

Four Interchangeable Sequi-Gen GT IPC sizes.

Integral Plate Chamber

The upper buffer chamber extends over the entire area of the bonded gel plate, making contact with the top of the gel near the fill spout.

Ever have your students sequence a gene? Now you can! The long, thin polyacrylamide gel format of the Sequi-Gen GT cell provides optimal separation of long and short DNA or RNA fragments that differ by only a single nucleotide. In addition to DNA sequencing, this system is useful for a wide array of applications:

Differential display
Microsatellite analysis
DNA fingerprinting and footprinting
Single-strand conformation polymorphism (SSCP) studies
Heteroduplex analysis
RNase protection assays
S1 nuclease mapping
Primer extension analysis
DNA/protein binding studies (gel shift assays)
Oligonucleotide analysis

The patented Sequi-Gen GT nucleic acid sequencing cell makes casting and running sequencing gels easy. The unique Sequi-Gen GT cell ensures smile-free DNA sequence and fingerprinting data and has IEC 1010 safety certification — the most rigorous international safety standard — to ensure the highest personal and environmental protection.

Key features:

Simple, problem-free horizontal gel casting
Unique design for heat dissipation maintains uniform temperature and eliminates smile patterns
Easy-to-use, lever-operated clamps facilitate rapid assembly and gel casting without tape and grease
Modular design includes a universal transparent base for all four Sequi-Gen sizes
Convenient drain port in the sealed upper buffer chamber allows safe and easy buffer drainage
Comprehensive selection of combs and spacers meet all application demands