Restriction Digestion and Analysis of Lambda DNA Kit

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Overview

Purchasing Guide Table
Purchasing Guide Table

Reduce. Reuse. Refresh.

Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase.


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Lab Preparation Checklist Lab Preparation Checklist
Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
HindIII restriction enzyme 1
Pstl restriction enzyme 1
EcoRI restriction enzyme 1
Restriction buffer 1
Lambda DNA, uncut 1
DNA size standard (HindIII lambda DNA digest) 1
Sample loading buffer, 5x, 1 ml 1
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 60
Foam floats 8
Gel staining trays 4
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Horizontal gel electorphoresis chambers 4–8
Adjustable micropipets
    2–20 µl 4–8
    20–200 µl 1
Pipet tips
    2–200 µl, BR-35 1 bag
Power supplies 2–4
Recommended (Optional) Accessories:
Water bath or dry bath
Rocking platform
Gel support film
Microwave oven
DNA Analysis Flowchart DNA Analysis Flowchart
Restriction Digestion and Analysis of Lambda DNA Flowchart
Key Kit Features Key Kit Features
Key Kit Features
  • Grow a bacterial library
  • Clone a gene of interest in bacteria
  • Express a protein of interest in bacteria
  • Purify a protein of interest from bacteria
  • Take a protein to market
  • Complete in four 45 min lab sessions
Curriculum Fit Curriculum Fit
Curriculum Fit
Lambda Bacteriophage Lambda Bacteriophage
Lambda DNA gel
Lambda DNA Gel

Lambda DNA gel has been a workhorse of molecular biology for decades. It is vital in the fields of molecular cloning and genomic sequencing since it can be used to subclone very long genomic DNA fragments much more efficiently than plasmid vectors. Lambda DNA comes from a bacterial virus, or bacteriophage, which attacks bacteria by injecting them with its nucleic acid. Once inside, Lambda DNA hijacks the bacterial cellular machinery and replicates itself until the cells burst, releasing millions more bacteriophage to carry out the same infection process. Bacteriophage lambda is harmless to humans and other eukaroytic organisms, and therefore makes an excellent source of DNA for experimental study.

The lambda genome has approximately 48,000 base pairs. Each restriction enzyme used in this kit will cut the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The three different sets of DNA fragments that result are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast DNA stain.

Large Class Preparation Guide
Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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Complete Purchasing Guide
Complete Purchasing Guide

Download the complete Biotechnology Explorer™ Refresh Kit Components Purchasing Guide.


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 Bulletin 5872

Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of three different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.

Features and Benefits

  • Aligns with AP Lab 6
  • Teaches about using genetic engineering and restriction enzymes as microbiology tools

With this kit, students are able to:

  • Use agarose gel electrophoresis to study digestion patterns
  • Generate standard curves from series of DNA size standards
  • Analyze migration distances to determine DNA fragment sizes

Students learn the procedures and principles of restriction digestions and DNA gel electrophoresis, including:

  • Agarose gel casting
  • Sample loading
  • Size-based separation of DNA fragments
  • DNA staining
  • Graphic analysis

How It Works

The lambda genome has approximately 48,000 base pairs. Each restriction enzyme used in this kit cuts the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes. The three different sets of DNA fragments that result from the enzymatic digestion are separated by agarose gel electrophoresis and visualized using Bio-Rad's safe Fast Blast™ DNA stain. Banding patterns from each sample are then compared to each other and to a DNA size standard. Students use their electrophoresis results to construct standard curves and determine the precise DNA fragment sizes generated by the different restriction enzymes.

More Information

  • Two-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
HindIII restriction enzyme 1
Pstl restriction enzyme 1
EcoRI restriction enzyme 1
Restruction buffer 1
Lambda DNA, uncut 1
DNA size standard (HindIII lambda DNA digest) 1
Sample loading buffer, 5x, 1 ml 1
Agarose powder, 5g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 60
Foam floats 8
Gel staining trays 4
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Restriction Digestion and Analysis of Lambda DNA Kit

166-0002EDU
DNA restriction analysis classroom study kit, includes DNA, restriction enzymes, buffers, agarose, Fast Blast DNA stain, curriculum, and more, for 32 students; education use only

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Number Description Options
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download
3055 Restriction Digestion and Analysis of Lambda DNA Kit, Biotechnology Explorer, Quick Guide Please login to download this document
4006102 Instruction Manual, Restriction Digestion and Analysis of Lambda DNA Kit, Biotechnology Explorer, Rev D Please login to download this document [ Add to Cart ]
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide, Rev A Click to download
5872 Biotechnology Explorer Refresh Kit Components Purchasing Guide, Rev A Click to download
5914 DNA Staining Using Fast Blast DNA Stain or SYBR Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software, Rev A Click to download