Higher sensitivity and better transfer efficiency is seen with the Trans-Blot® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.
Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.
See how the Trans-Blot Turbo system measures up against traditional blotting techniques.
See how the Trans-Blot Turbo system performs compared to the iBlot dry blotting system.
Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs
E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.
2x Protein Transfer
Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.
The Trans-Blot Turbo transfer system combines traditional blotting techniques with modern filter paper and buffers, allowing rapid transfer of proteins from gels to membranes with minimal preparation time. The system integrates a high amperage power supply that directs current between a built-in platinized titanium anode and a stainless steel cathode. This enables highly efficient and rapid transfer in as little as 3 minutes. The entire system is in a ready-to-use format that allows researchers to obtain their results faster and easier, with reproducibility that is difficult to achieve using traditional blotting methods.
170-4155EDU Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit; education use only
170-4156EDU Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system; education use only
170-4157EDU Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system; education use only
170-4158EDU Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system; education use only
170-4159EDU Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system; education use only
166-2875EDU Comprehensive protein classroom study kit, includes Protein Profiler Module (#166-2700EDU), Western Blot Module (#166-2800EDU), Trans-Blot® Turbo™ mini nitrocellulose transfer packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free™ gels (10), application note, for 32 students; education use only
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