GS HBsAg EIA 3.0

Earliest Hepatitis B Infection Detection Demonstrated on
21 Commercial Seroconversion Panels*

* The sensitivity of GS HBsAg EIA 3.0, procedures A and B, was assessed using 20 seroconversion panels purchased from Boston Biomedica, Inc. (West Bridgewater, MA), and 1 panel from Serologicals, Inc. (Clarkston, GA). The number of the bleed at which HBsAg was detected (S/C ≥ 1.0) by the GS HBsAg EIA 3.0, procedures A and B, is compared to other licensed HBsAg assays (as recorded in the certificates of analysis).

GS HBsAg 3.0 Static Procedure

• Earlier detection on 12 out of 19 seroconversions for HBsAg 3.0 versus competitor assay number 1
• Earlier detection on 16 out of 20 seroconversions for HBsAg 3.0 versus competitor assay number 2

GS HBsAg 3.0 Shaking Procedure

• Earlier detection on 15 out of 19 seroconversions versus competitor assay number 1
• Earlier detection on 18 out of 20 seroconversions versus competitor assay number 2

GS HBsAg EIA 3.0 Testing Procedure

Test Format +			Sample/Reagent Addition Monitoring
		Microplate coated with monoclonal antibody (Ab)	Color of well BEFORE addition		Color of well AFTER addition
		100 µL of control or sample Incubate 60–65 minutes at 37°C
Wash
		100 µL of Working Conjugate Solution Incubate 60–65 minutes at 37°C
Wash
		100 µL of Working TMB Solution Incubate 30 minutes at room temperature
		100 µL of Stopping Solution
READ within 30 minutes at 450 nm with the 615–630 nm filter as a reference
Anti-HBsAg Monoclonal Ab   HBsAg   Anti-HBsAg Monoclonal Ab Conjugate + * Static or Shaker Reactive samples after 30 minute incubation

Serology Profiles

Hepatitis B: Example of Acute Hepatitis

Comprehensive Product Line for Hepatitis Testing

Fast incubation time: 2.5 hours		Standardized procedure for all Bio-Rad assays		Microplate strip identification
Common reagents for all Bio-Rad kits		Color-coded reagent and kit labels		Color changes during protocol steps
Manual and automated use		Barcoded reagents		Controls with color-coded caps