PR 3100 TSC Microplate Reader

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Overview

Reference Guide for Daily Operation
PR3100 Touch Screen Control (TSC)

PR3100 TSC Quick Reference Guide

Refer to the PR3100 TSC User Manual and PR3100 TSC Microplate Assay Testing Reference Manual (MATRM) for more detailed instructions.

Daily Operation

1. Daily Start-up (Power on)
(Stand clear minimum of 8 inches before powering on reader)
a. Power on Reader with switch on the rear right. Log in using User Name and Password. Touch [OK].
b. Allow one minute to load assay files from the APF USB stick.

2. Create Sample ID List
(applies only for methods with v2.0 – see Table 3 in Section 9 of the MATRM)
a. Touch [Create/Edit a Sample ID List].
b. Highlight the assay under Filename and touch [»].
c. Touch [Manual enter sample ID list…].
d. Touch “sm1” well then [Edit] box.
e. Scan first bar code label. It will advance to next available well. Scan in remaining bar code labels.
f. Touch [OK] and review plate map. If correct, touch [OK] and [»] to proceed to Save as screen.
g. Save using MMDDYY_run#_method convention.

3. Read “Preprogrammed Assay Plate”
a. Touch [Start Measurement] box.
b. Touch radio button for User Predefined Method or Start Favorite.
c. Select Assay File Name and touch [»].
d. Enter Workspace Name incorporating Date, Lot # and Kit Exp Date (i.e., 071207,BB725,122607). For plate that does not utilize Sample ID List created in step 2, skip to step g.
e. Touch [Insert…] to input the Sample ID List created for this particular run. (Saved in step 2,g).
f. If Quantitative or Semi-quantitative Assay requires change in standard value, touch [Modify Layout] box.
g. Place plate in the plate support tray.
h. Touch [Finish].
i. If assay requires any other input such as Min/Max control value or verification of valid BR Anti-HBs (0–400), an [Edit Prompt] box will appear. Otherwise, it will start shaking the plate for 20 seconds outside the reader before taking in the plate to read.
j. Result will print automatically if the plate meets all the validation criteria.
k. Verify that all validations have passed.
l. Verify patient results are within defined specifications.
m. If any validation is not met, the result report can be printed without the Interpretation.
  • Touch [Yes] button when the QC Validation Criteria Failed box appears with failed validation criteria asking to proceed or to stop evaluation.
  • Result will print automatically.

4. Read “Raw Absorbance of Plate”
a. Touch [Start Measurement] box.
b. Touch radial button for Obtain Raw Data and Touch [»].
c. Touch [Measurement Parameters] to access the [Meas Params] folder.
d. Select single or dual wavelength(s) for reading by selecting the wavelength(s) to be read.
e. Touch [Shaking] box to input shake options.
f. Touch [OK] and [»].
g. At Start Measurement Screen, enter workspace name.
h. Place plate in the plate support tray.
i. Touch [Finish] box.
j. Result can be viewed by touching [View Data] box on or printing by touching [Print] box.
k. Touch [»] to save the .wsp file. Remarks can be entered in Remarks box.
l. Touch [Finish] to get back to Wizard List screen.

5. Daily Shutdown — Do Not Leave Plate in Reader
a. Take plate off the Plate Support.
b. Inspect reader for any spill and clean as needed if spill occurs.
c. Touch [Exit Magellan] box.
d. When the Popup message “It is now ok to turn off Instrument” comes up, Power down Reader with switch on the rear right.


6. Power Off the PR3100 TSC BeforeTaking Out the Export USB Stickto Access the ASCII or ASTM File

a. Insert the Export USB stick into a USB port on a PC and open using appropriate software. It can be opened with standard text file program, EXCEL or ASTM interface program.
b. When inserting back to PR3100 Reader, make sure the reader is powered off.
Reference Guide for QC Pac2
PR3100 Touch Screen Control (TSC)

PR3100 TSC Quick Reference Guide

Refer to the PR3100 TSC User Manual and PR3100 TSC Microplate Assay Testing Reference Manual (MATRM) for more detailed instructions.

QC Pac2 – Reader Verification

1. Installing the QC Pac2 USB Stick
(Stand clear minimum of 8 inches before powering on reader)
a. Power down Reader with switch on the rear right.
b. Take the APF USB stick out from the back of Touchscreen compartment. There is a sliding door to the rear right of the Touchscreen.
c. Insert the QC Pac2 USB stick into the back of Touchscreen into the USB port compartment. (Make sure the small light hole on the USB stick is facing down.)

2. Running the QC Pac2
a. Touch [Q2] box on the top toolbar.
b. Follow instruction on screen. Touch [»].
c. Enter User Name.
d. Enter QC Plate #.
  • Enter #, first time the plate is used.
  • Touch [⇓] to show previous Plate # to be selected.
e. Place the Verification Plate on the plate support with marking A1 on the plate in the top left corner.
f. Touch [»]. Reader will start reading the plate.
g. When the plate comes out and stays out, turn the plate 180° with marking A1 at the bottom right corner.
h. Touch [»]. Reader will start reading the plate.
i. When the plate comes out and stays out for the second time, take the plate off the plate support and place it back into the plastic bag before putting back into the case.
j. Touch [Move…] box to access plate movement.
k. Retract Plate Support by touching [In].
l. Touch [Finish] to go to save result option — default filename contains the Date in the filename.
m. Touch [OK] and Save.
n. Review screen for any number other than “0” next to FAIL. “0” means all passed.
o. Touch [Printer Icon] to print the complete report. Select the printer driver. Default is PCL Compatible Printer (Color).
p. Touch [OK] to print complete report.
q. Touch File on Toolbar followed with Exit.
r. Power down Reader with switch on the rear right.
s. Take out the QC Pac2 USB stick and replace with the APF USB stick.
t. Ready for Daily Start-up.
Reference Guide for Magellan Operators
PR3100 Touch Screen Control (TSC)

PR3100 TSC Quick Reference Guide

Refer to the PR3100 TSC User Manual and PR3100 TSC Microplate Assay Testing Reference Manual (MATRM) for more detailed instructions.

Software Navigation for Magellan Operators: Wizard List Screen

Start Measurement
Obtain Raw Data
Measurement Parameters box (Required to be entered)
  • General Tab: General Information
  • Meas Param…: Measurement and Reference wavelength options
    — Read Mode options (Normal, Accuracy or Center)
  • Shaking: Shaking options
Use Predefined Method
All predefined methods
  • Enter Modify Layout for Quant and Semi Quant assays (in Start Measurement screen)
Start Favorite
15 Selected methods
  • Enter Modify Layout for Quant and Semi Quant assays (in Start Measurement screen)

Evaluate Results
Select a File
  • View data
  • Print (reprint)

Create/Edit a Sample-ID List
(Applies only to the 22 methods with v2.0 in Remarks section when selecting method to run.)

Plate In/Out
Moves plate In or Out

Instrument Control
Movements (Plate and Filter)
  • Moves plate In or Out
  • Moves Absorbance Filter slide In or Out
Handheld Bar Code Scanner
  • Allows to run Handheld
  • Bar code scanner test
PC Remote (Not applicable to Bio-Rad Configuration)

Change User
Allows User change without rebooting PR3100 TSC

Options
Touchscreen
  • Date, Time, Contrast and Keyboard (choice of regular or large keyboard)
Printer
  • Printer driver selection
  • Font size
  • USB Port selection
  • Letter Paper Size selection
  • Portrait Orientation selection
Plate View (Not applicable to Bio-Rad Configuration)
Misc
  • Wizard screen options
  • Start-up screen options
  • Audit Trail
  • Change password…
  • Language (Do Not Change. Conflicts with Preprogrammed Bio-Rad Assays.)
  • Add HUID (Do Not Change. Conflicts with Preprogrammed Bio-Rad Assays.)

About Magellan
Information on this Instrument Software

Exit Magellan
Allows to power down

The compact PR 3100 TSC is designed to measure the absorbance of specimens in 96-well microplates. It is configured with five pre-installed filters that are compatible with Bio-Rad blood virus, autoimmune, and infectious disease testing. The on-board, fully functional computer provides comprehensive data reduction. The PR 3100 TSC can perform automated sample barcode reading, along with data export via a USB thumb drive.

Number Description Options
R-774 PR3100 TSC Microplate Reader Product Sheet Click to download [ Add to Cart ]
R-776 PR3100 TSC Quick Reference Guide Click to download [ Add to Cart ]