iScript™ Select cDNA Synthesis Kit #170-8896

Packaging Options

• Catalog #170-8896, 25 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
• Catalog #170-8897, 100 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
• Catalog #170-8897BUN, 500 x 20 µl reactions, cDNA synthesis kit, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water

Description

The iScript Select cDNA synthesis kit is a sensitive reverse transcription system offering flexible priming strategies. Unlike our iScript cDNA synthesis kit and iScript reverse transcription supermix for RT-qPCR, this kit comes with multiple reaction components (5x buffer, oligo[dT], random primers, proprietary gene-specific primer enhancer solution, and water) that must be combined during reaction setup.

Key Features and Benefits

• Have flexibility in mRNA priming strategy — oligo(dT), random primer, and gene-specific primer enhancer solution provided in separate tubes
• Synthesize long cDNAs up to 8 kb — quality controlled for reliable synthesis of cDNA >6 kb in length
• Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
• Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription