iScript™ One-Step RT-PCR Kit for Probes #170-8895

Packaging Options

• Product catalog #170-8894, 50 x 50 µl reactions, one-step real time RT-PCR kit, includes iScript reverse transcriptase, 2x probes RT-PCR reaction mix, nuclease-free water
• Product catalog #170-8895, 200 x 50 µl reactions, one-step real time RT-PCR kit, includes iScript reverse transcriptase, 2x probes RT-PCR reaction mix, nuclease-free water

Description

The iScript™ one-step RT-PCR kit for probes is a highly sensitive solution for one-step real-time quantitative RT-PCR on a broad range of thermal cyclers. cDNA synthesis and PCR amplification are conveniently carried out in the same tube, using the powerful combination of iScript RNase H+ reverse transcriptase and hot-start iTaq™ DNA polymerase.

Key Features and Benefits

• Maximize throughput and reduce the contamination risk of RT-qPCR analysis — 1-step RT-qPCR protocol allows quick and easy screening of a large number of samples
• Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total RNA input (1 µg—1 pg) with a highly efficient MMLV-RT RNaseH+
• Benefit from superior compatibility with hybridization probe chemistry — optimized for sensitive detection using all types of hybridization probes, including dual-labeled oligonucleotide probes, FRET probes, and molecular beacons
• Prevent non-specific amplification and primer dimers — iTaq antibody-mediated hot-start DNA polymerase contains an antibody that sequesters activity prior to the initial PCR denaturation step
• Avoid skewed data from RNA degradation — potent blend of RNaseA inhibitor protects RNA during setup and reverse transcription