iQ™ SYBR® Green Supermix #170-8882

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12.5 ml (10 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 500 x 50 µl reactions

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Number Description Options
170-8880-MSDS iQ™ SYBR Green Supermix English

Packaging Options

  • Catalog #170-8880, 2.5 ml (2 x 1.25 ml vials), 100 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein
  • Catalog #170-8882, 12.5 ml (10 x 1.25 ml vials), 500 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein
  • Catalog #170-8884, 25 ml (20 x 1.25 ml vials), 1,000 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein
  • Catalog #170-8884BUN, 50 ml (40 x 1.25 ml vials), 2,000 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein
  • Catalog #170-8885, 50 ml (1 x 50 ml bottle), 2,000 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein
  • Catalog #170-8886, 25 ml (5 x 5 ml vials), 1,000 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein
  • Catalog #170-8887, 50 ml (10 x 5 ml vials), 2,000 x 50 µl reactions, 2x qPCR mix, contains dNTPs, 50 U/ml antibody-mediated hot-start iTaq DNA polymerase, 6 mM MgCl2, SYBR® Green I, enhancers, stabilizers, 20 nM fluorescein

Description

iQ™ SYBR® Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR). It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers, and fluorescein.

iQ™ SYBR® Green supermix lets you:

  • Perform gene expression analysis with increased sensitivity and efficiency
  • Efficiently amplify a variety of templates (cDNA, genomic DNA, and plasmid DNA)

iQ™ SYBR® Green supermix contains:

  • Antibody-mediated hot-start iTaq DNA polymerase for quick activation and higher specificity
  • Fluorescein for use as a passive reference dye for well factor collection (on iQ™, iQ™5, MyiQ™, and MyiQ™2 real-time PCR detection systems)
  • Optimized buffer formulation for reliable results

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection