iScript™ Advanced cDNA Synthesis Kit for RT-qPCR #170-8842

Packaging Options

• Catalog #170-8842, 50 x 20 µl reactions, reverse-transcription reagent kit, includes 200 µl 5x iScript advanced reaction mix, 50 µl iScript advanced reverse transcriptase
• Catalog #170-8843, 250 x 20 µl reactions, reverse-transcription reagent kit, includes 1,000 µl 5x iScript advanced reaction mix, 250 µl iScript advanced reverse transcriptase

Description

The iScript advanced cDNA synthesis kit for RT-qPCR is an enhanced formulation that delivers increased data throughput from a single 20 µl reverse transcription (RT) reaction for real-time qPCR. This two-tube kit enables superior RT capacity of input RNA up to 7.5 µg.

Key Features and Benefits

• Increase qPCR data throughput and cost effectiveness from a single reaction — cDNA synthesized from higher input RNA amounts can be used for analyzing large numbers of target genes
• Detect low-level target genes — uncompromised sensitivity even with less input RNA for when RNA sample is limited
• Reduce inter-assay variability and improve reproducibility — eliminate the need for multiple cDNA synthesis reactions
• Allow true representation of target mRNA levels — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and RT
• Increase primer design flexibility and prevent 5' and 3' bias — optimum blend of oligo(dT) and random primers for superior RNA coverage
• Set up reactions with ease and obtain cDNA in less time — 2-tube kit generates cDNA in 35 min