Bio-Safe Coomassie Stain #161-0786

SDS-PAGE Mini Gels

1. Wash the gels 3 times for 5 min each in 200 ml of ddH₂O.
2. Remove all the water form the staining container and add 50 ml of Bio-Safe™ Coomassie stain or enough to completely cover the gel.
   
   Gently shake for 1 hr. Protein bands will be visible within 20 min and reach maximum intensity within 1 hr.
   
   Longer incubations will not increase the background.


3. Rinse the gels in 200 ml of ddH₂O for at least 30 min. Stained gels can be stored in water.

Note: Residual SDS in the gel may cause background staining and interfere with band intensity while the gel is in the stain. Rinsing the gel extensively in water after staining will remove background and allow proper visualization of the bands.

SDS-PAGE Gels
For large gels (16–20") increase the volumes in the above method twofold.

Peptide Gels
Fix gels in 40% methanol, 10% acetic acid for 30 min. Then proceed with staining from step 2 of the SDS-PAGE Mini Gels section. Extend step 3 to 2 hr.

Note: Peptide bands will not be clearly visible until after the final wash.