Precision Plus Protein™ WesternC™ Standards #161-0376

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Precision Plus Protein™ WesternC™ Standards

250 μl, 10 blue-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications

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MW size range, kD 10–250
Stain type Combination of stained Dual Color and unstained
Volume, µl 250
Total number of applications 50
Shelf life at –20°C, months 12
Loading volume on mini gels, µl for chemiluminescent detection, 5. Precision Protein™ StrepTactin-HRP or -AP conjugate needed.
Loading volume on mini gels, µl for fluorescent blot detection, 5 of 1:10 dilution
Application Type  
Monitoring electrophoretic separation for SDS-PAGE gels Yes
Molecular weight sizing on SDS-PAGE gels Yes
Monitoring blot transfer efficiency Yes
Fluorescence blots Yes
Molecular weight sizing on Blots
Loading Volume on Mini Gels  
For Coomassie staining, µl N/A
For stain-free gels, µl N/A
For silver staining, µl N/A
For SYPRO Ruby staining, µl N/A
For blot development, µl N/A
StrepTactin conjugate working dilutions for colorimetric blot development N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development N/A
Number Description Options
161-0376-MSDS Precision Plus™ Western C Protein Standards English

The Precision Plus Protein™ WesternC™ standards are a combination standard for western blotting or fluorescence detection. They contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. For blot development, Precision Protein™ StrepTactin-HRP conjugate (#161-0380) or Precision Protein StrepTactin-AP conjugate (#161-0382) should also be ordered. For fluorescence detection, no additional product is needed.

Applications and Uses

Choose Precision Plus Protein WesternC standards for SDS-PAGE and blotting:

  • Monitoring electrophoretic separation
  • Monitoring blot transfer efficiency with easy orientation via multicolor standards
  • Molecular weight sizing of proteins after blot development
  • Fluorescent blot detection with prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range (prepare a 1:10 dilution of the WesternC standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection)

More Information

Loading buffer:
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue