Precision Plus Protein™ All Blue Standards #161-0373

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Precision Plus Protein™ All Blue Standards

500 μl, mixture of 10 blue-stained recombinant proteins (10–250 kD), including 3 reference bands (25, 50, 75 kD), 50 applications

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MW size range, kD 10–250
Stain type All Blue
Volume, µl 500
Total number of applications 50
Shelf life Store for 6 months at 4°C or 12 months at –20°C
Loading volume on mini gels, µl for Coomassie staining, 10
Loading volume on mini gels, µl for fluorescent blot detection, 5 of 1:10 dilution
Application Type  
Monitoring electrophoretic separation for SDS-PAGE gels Yes
Molecular weight sizing on SDS-PAGE gels Yes
Monitoring blot transfer efficiency Yes
Fluorescence blots Yes
Loading Volume on Mini Gels  
For Coomassie staining, µl N/A
For stain-free gels, µl N/A
For silver staining, µl N/A
For SYPRO Ruby staining, µl N/A
For blot development, µl N/A
StrepTactin conjugate working dilutions for colorimetric blot development N/A
StrepTactin conjugate working dilutions for chemiluminescence blot development N/A
Number Description Options
161-0373-MSDS Precision Plus™ All Blue Protein Standards English

Precision Plus Protein All Blue standards are a mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, and 75 kD).

Applications and Uses

Choose Precision Plus Protein All Blue standards for:

  • Monitoring electrophoretic separation
  • Molecular weight sizing on SDS-PAGE gels
  • Monitoring blot transfer efficiency
  • Fluorescent blot detection in the red wavelength range (prepare a 1:10 dilution of the All Blue standard and load 5 µl per well)

More Information

Loading buffer:
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue

Store for 6 months at 4°C or 12 months at –20°C.