IEF Standards #161-0310

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250 μl, pI range: 4.45–9.6, phycocyanin, β-lactoglobulin B, bovine and human CA, equine myoglobin, human hemoglobin A and C, lentil lectin, and cyt C

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161-0310-MSDS IEF Standards pI 4.45-9.6 English

The IEF standards are a mixture of nine proteins (pI 4.45–9.6 kD). Five of the nine proteins are naturally colored, allowing continuous monitoring of the focusing process. The standards are provided in a ready-to-use aqueous solution.

Applications and Uses

Choose IEF standards for:

  • PI calibration in analytical polyacrylamide or agarose IEF gels.

More information

Size Range Quantity Recommended Load Volume
9 proteins, pI 4.45–9.6 16 mg protein in 250 µl of 50% (v/v) glycerol with 0.02% NaN3 Mini gels: 3 µl (Coomassie R-250 stain/crocein scarlet); 0.5 µl (silver stain)