Bio-Plex Pro™ Human Th17 Cytokine Assays

The Bio-Plex Pro™ human Th17 cytokine panel is a unique blend of magnetic bead–based immunoassays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammatory conditions such as:

• Rheumatoid arthritis, lupus, psoriasis, multiple sclerosis, and other autoimmune diseases
• Antimicrobial immunity
• Transplant rejection
• Cancer

The panel enables measurement of the following analytes in diverse matrices such as serum, plasma, and cell culture supernatants:

• IL-1β
• IL-4
• lL-6
• IL-10
• IL-17A
• IL-17F
• IL-21
• IL-22
• IL-23
• IL-25
• IL-31
• IL-33
• IFN-γ
• sCD40L
• TNF-α
• IL-17A/F (singleplex only)

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

• Reagent kit ll with diluents optimized for serum and plasma samples
• Assay quick guide to get you started right away
• Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
• Includes premixed standards for generation of standard curves over a broad dynamic range for each target
• 2-level quality controls included with premixed kits

Flexible Ordering Options

• Premixed panel — complete, ready-to-use 15-plex Bio-Plex Pro human Th17 cytokine panel and 3-plex Bio-Plex Pro TGF-β assay kits
• Human Th17 singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires reagent kit ll (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)
• Custom premixed assays (We Mix) — includes 2-level quality controls, available as Bio-Plex^® x-Plex™ assays built using the Bio-Plex^® Assay Builder
• Custom assays (You Mix) — available as Bio-Plex Express assays built using the Bio-Plex Assay Builder

Additional Th17 Pathway Assays:

• TGF-β 3-plex panel (#171-V4003M)
• TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-D60001) for a complete assay