Criterion™ Blotter

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Overview

Trans-Blot® Turbo Transfer System
New Product

 

Trans-Blot® Turbo™ Transfer System

The Trans-Blot Turbo system is a fast, efficient, and reproducible transfer system for transferring proteins from gels to membranes in as little as 3 minutes.

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Immun-Blot® LF PVDF
New Product


Immun-Blot® Low Fluorescence PVDF Membrane

Bio-Rad’s Immun-Blot LF PVDF membrane offers the same benefits as standard PVDF membranes in addition to minimal autofluorescence and high sensitivity, making it the premium choice for fluorescent and multiplex fluorescent immunoblotting needs.

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Selection Guide
Protein Blotting Cell Selection Guide
Tank Transfer Semi-Dry Transfer
Mini Trans-Blot Cell Criterion Blotter Trans-Blot Cell Trans-Blot Plus Cell Semi-Dry Transfer Trans-Blot SD Cell
Blotting area (W x L) 10 x 7.5 cm 15 x 9.4 cm 16 x 20 cm 28 x 26.5 cm 24 x 16 cm
Transfer Parameters
Number of cassettes 2 2 1 for high-intensity transfer
2 with cooling
3 without cooling		 3 -
Buffer requirement 450 ml 1.3 L 2.5 L 10–12 L ≤200 ml
Electrode distance 4 cm 4.3 cm Flexible; 2 positions, 4 and 8 cm Flexible; 3 positions, 4, 7 and 10 cm Determined by sandwich thickness
Transfer time (high-intensity) 60 min 30 min (plate electrodes)
60 min (wire electrodes)		 30 min (plate electrodes)
60 min (wire electrodes)		 15–60 min 15–60 minutes
Cooling Bio-Ice cooling unit and/or cooling coil Sealed ice block Super cooling coil Super cooling coil -
Gel Capacity
25.6 x 23 cm (PROTEAN Plus Dodeca cell) - - - 1 gel per cassette
3 cassettes total
(all sizes)		 -
25 x 20.5 cm - - - -
20 x 20.5 cm - - - -
18.5 x 20 cm (PROTEAN II XL cell) - - - 1 gel per cassette
3 cassettes total
(all sizes)		 -
18.3 x 19.3 cm - - - 1 gel
(2 gels with stacking)
16 x 20 cm (PROTEAN II xi cell) - - 1 gel per cassette (both sizes)
Standard transfer: 3 cassettes
High-intensity transfer: 1 cassette		 1 gel per cassette
3 cassettes total		 1 gel
(2 gels with stacking)
(2 gels with stacking)
16 x 16 cm - -
13.3 x 8.7 cm (Criterion cells) - 1 gel per cassette
2 cassettes total		 2 gels per cassette
Standard transfer: 3 cassettes
High-intensity transfer: 1 cassette		 4 gels per cassette
3 cassettes total		 3 gels side-by-side
8.3 x 7.3 cm (Mini-PROTEAN Tetra cell) 1 gel per cassette
2 cassettes total
(both sizes)		 2 gels per cassette
2 cassettes total
(both sizes)		 4 gels per cassette (both sizes)
Standard transfer: 3 cassettes
High-intensity transfer: 1 cassette		 9 gels per cassette
3 cassettes total
(both sizes)		 4 gels side-by-side
4 gels side-by-side
8.6 x 6.8 cm
Components
Criterion Components
Criterion Components
Criterion Transfer Conditions
Criterion Transfer Conditions
  20% Methanol 10% Methanol 15% Ethanol
Criterion Blotter
with plate electrodes 		100 V
30 minutes 		100 V
30 minutes 		Not recommended*
Criterion Blotter
with wire electrodes 		100 V
60 minutes 		100 V
30 minutes 		Not recommended*

* Our tests show only 60% transfer of E. coli proteins in 1 hour at 100 V. The ethanol buffer might work if longer transfers are acceptable or if your target protein transfers under this condition.

Assembly Tray
Assembly Tray
Assembly Tray

Included assembly tray and roller — use the assembly tray for gel soaking, for sandwich assembly, and to ensure correct orientation of gel and blot.

The Criterion™ blotter provides a combination of blotting efficiency and flexibility in an easy-to-use unit.

Features and Benefits

  • Capacity to blot 2 Criterion gels or 4 Mini-PROTEAN® handcast or precast gels
  • Closely spaced electrodes produce maximum field strength and efficient transfers in 30 min to 1 hr for most proteins; overnight transfer at lower voltages is also an option
  • Included assembly tray can be used for gel soaking, sandwich assembly, and to ensure correct orientation of the gel and blot
  • Included roller removes air bubbles in the sandwich to ensure complete transfer
  • Included sealed cooling unit is sufficient for most transfers and absorbs heat generated during rapid transfers
  • Optional cooling coil is available for applications that require precise temperature control
  • Cassette handles allow easy removal from the tank, and overflow chambers keep your laboratory bench dry

Applications and Uses

  • Western blotting
  • Nucleic acid transfer

To find a blotting package that fits your needs, see the Selection Guide.

Maximum gel size (W x L), cm 15 x 9.4
Buffer requirement, L 1.3
Gel capacity 4 Mini-PROTEAN® precast or handcast gels, or 2 Criterion™ precast gels
Recommended power supply PowerPac™ HC
Dimensions (W x L x H), cm 21.8 x 11.8 x 15
Weight, kg/lb 1.3/2.9
Criterion Blotter With Plate Electrodes #170-4070
Criterion™ Blotter With Plate Electrodes

170-4070
Protein blotting cell, includes 2 gel holder cassettes, absorbent filter paper, 4 foam pads, gel/blot assembly tray, roller, sealed ice block, lid/cables

List Price:   $775.00
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Criterion Blotter With Wire Electrodes #170-4071
Criterion™ Blotter With Wire Electrodes

170-4071
Protein blotting cell, includes 2 gel holder cassettes, absorbent filter paper, 4 foam pads, gel/blot assembly tray, roller, sealed ice block, lid/cables

List Price:   $735.00
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Criterion Cell/Plate Blotter System #165-6024
Criterion™ Cell/Plate Blotter System

165-6024
Vertical midi-gel electrophoresis cell (#165-6001) and Criterion blotter (#170-4070) with plate electrodes

List Price:   $1,255.00
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Criterion Cell/Wire Blotter System #165-6025
Criterion™ Cell/Wire Blotter System

165-6025
Vertical midi-gel electrophoresis cell (#165-6001) and Criterion blotter (#170-4071) with wire electrodes

List Price:   $1,255.00
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Criterion Blotter With Plate Electrodes and PowerPac HC Power Supply #170-3872
Criterion™ Blotter With Plate Electrodes and PowerPac™ HC Power Supply

170-3872
100–120/220–240 V, plate blotter with HC power supply (#164-5052), includes lid, cables, gel holder cassettes, blot paper, foam pads, gel/blot tray, roller, sealed ice block

List Price:   $1,725.00
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Criterion Blotter With Wire Electrodes and PowerPac HC Power Supply #170-3874
Criterion™ Blotter With Wire Electrodes and PowerPac™ HC Power Supply

170-3874
100–120/220–240 V, wire blotter with HC power supply (#164-5052), includes lid, cables, gel holder cassettes, blot paper, foam pads, gel/blot tray, roller, sealed ice block

List Price:   $1,630.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
2558 Criterion Blotter Flier, Rev A. Click to download [ Add to Cart ]
2033 Western Blotting Product Brochure, Rev C Click to download [ Add to Cart ]
4006190 Instruction Manual, Criterion Blotter, Rev B Click to download [ Add to Cart ]