Precision Plus Protein™ Unstained Standards

Precision Plus Protein unstained standards on a gel. These standards have been engineered to contain the unique Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots.

Western blot detection of Green Fluorescent Protein (GFP) and unstained Precision Plus Protein standards using the Immun-Star horseradish peroxide (HRP) chemiluminescent detection kit. A gel was run with 0.5 µl of standards (lane 1) and a dilution series of E. coli lysate containing GFP (lanes 2–8), and was transferred to a PVDF membrane. The blot was probed with a primary antibody specific for GFP, then incubated simultaneously with goat anti-mouse-HRP and StrepTactin-HRP. After incubating in the Immun-Star HRP detection solution for 2 min, the blot was exposed to film for 10 min.

Western blot detection of Green Fluorescent Protein (GFP) and unstained Precision Plus Protein standards using the Immun-Blot alkaline phosphatase (AP) colorimetric detection Kit. Maximum sensitivity achievable with Immun-Blot AP is 100 pg. A gel run with 4 µl of standards (lane 1) and a dilution series of E. coli lysate containing overexpressed GFP (lanes 2–10) was transferred to a PVDF membrane. The blot was probed with a primary antibody specific for GFP, then incubated with StrepTactin-AP and a secondary antibody conjugated to AP. The blot was developed using the Immun-Blot AP kit.