Sub-Cell^® Model 192 Cell

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Overview

			UVTP Tray (cm)				Combs			PowerPac Basic Power Supply
Catalog #	Casting Gates	Gel Caster	25 x 10	25 x 15*	25 x 20	25 x 25	26-Well	51-Well	Comb Holder (1)	PowerPac Basic Power Supply
170-4508			•				•	•	•
170-4509	•			•			•	•	•
170-4510					•		•	•	•
170-4511						•	•	•	•
170-4504		•	•				•	•	•
170-4505	•	•		•			•	•	•
170-4506		•			•		•	•	•
170-4507		•				•	•	•	•
164-0306	•	•		•			•	•	•	•

* 25 x 10 cm trays allow casting gels in the cell using casting gates. Other gel sizes can be cast with a gel caster.

Sub-Cell Model 192 Comb Selection Guide

Sub-Cell^® Model 192 Adjustable-Height Combs

Catalog #	# of Wells	Height	Thickness	Width of Well	Length of Teeth	Volume (in 5 mm deep gel)
Adjustable-Height Combs for Sub-Cell Model 192 Systems*
170-4528**	51	—	0.75 mm	3 mm	15 mm	11.25 µl
170-4529**	51	—	1.5 mm	3 mm	15 mm	22.5 µl
170-4526**	26	—	0.75 mm	6 mm	15 mm	22.5 µl
170-4527**	26	—	1.5 mm	6 mm	15 mm	45 µl
170-4530	2 or 4 preparative	—	0.75 mm	46 or 98 mm	15 mm	172.5 or 367.5 µl
	2 reference			6 mm		22.5 µl
170-4528	2 or 4 preparative	—	1.5 mm	46 or 98 mm	15 mm	345 or 735 µl
	2 reference			6 mm		45 µl

* Combs for Sub-Cell Model 192 cells can be used with Sub-Cell Model 96 cells and vice versa. Adjustable-height combs require comb holder, catalog #170-4525. Each system includes 1 comb holder.
** Multichannel pipet–compatible.

Gel Casting Guide

Agarose Gel Casting Guide

Hand Casting Options for a Variety of Gel Sizes

Two accessories are available for hand casting with the Sub-Cell family of electrophoresis cells: gel casters and casting gates. Gel casters offer the flexibility of accommodating several different-sized gel trays. These are available for the entire line of Sub-Cell systems. Casting gates, available in specific sizes for each cell, offer the convenience of casting directly in the Sub-Cell cells.

Gel Caster Guide
Tray Size (cm)	Mini Gel Caster	Full-Size Gel Caster	Model 96 Gel Caster	Model 192 Gel Caster
7 x 7	•	•	•	•
7 x 10	•	•	•	•
15 x 7	•	•	•	•
15 x 10	•	•	•	•
15 x 15		•	•	•
15 x 20		•	•	•
15 x 25		•	•	•
25 x 10			•	•
25 x 15			•	•
25 x 20				•
25 x 25				•

Casting Guide
Electrophoresis Cell	Tray Size (cm)	Casting Gates	Gel Caster
Mini-Sub cell GT	7 x 7	•	•
Mini-Sub cell GT	7 x 10		•
Wide Mini-Sub cell GT	15 x 7	•	•
Wide Mini-Sub cell GT	15 x 10		•
Sub-Cell GT	15 x 10		•
	15 x 15	•	•
	15 x 20		•
	15 x 25		•
Sub-Cell Model 96	25 x 10	•	•
Sub-Cell Model 96	25 x 15		•
Sub-Cell Model 192	25 x 10		•
	25 x 15	•	•
	25 x 20		•
	25 x 25		•

Sub-Cell Selection Guide


	Mini-Sub Cell GT*	Wide Mini-Sub Cell GT**	Sub-Cell GT	Sub-Cell Model 96	Sub-Cell Model 192
Cell size (W x L x H)	9.2 x 25.5 x 5.6 cm	17.8 x 25.5 x 6.8 cm	18 x 40.5 x 9.4 cm	29 x 30 x 9 cm	29 x 40 x 9 cm
Gel tray sizes (OD) (W x L)	7 x 7 cm 7 x 10 cm	15 x 7 cm 15 x 10 cm	15 x 10 cm 15 x 15 cm 15 x 20 cm 15 x 25 cm	25 x 10 cm 25 x 15 cm	25 x 10 cm 25 x 15 cm 25 x 20 cm 25 x 25 cm
ReadyAgarose gels accommodated	Yes	Yes	No	No	No
Sample throughput	8–30***	10–60***	1–120†	24–96***	24–192†
Base buffer volume	~270 ml	~650 ml	~1 L	~2 L	~3 L
Buffer recirculation	No	No	No	Yes	Yes
Bromophenol blue migration	~4.5 cm/hr (at 75 V)	~4.5 cm/hr (at 75 V)	~3.0 cm/hr (at 75 V)	~6.2 cm/hr (at 200 V)	~5.2 cm/hr (at 200 V)
* Mini ReadySub-Cell GT is a Mini-Sub cell GT dedicated to running ReadyAgarose precast gels, gel size 7 x 10 cm; sample throughput is 8, 12, or 2 x 8. This cell does not include casting gates, tray, or combs. Wide mini ReadySub-Cell GT is a wide Mini-Sub cell GT dedicated to running ReadyAgarose precast gels, gel size 15 x 10 cm; sample throughput is 20, 32, 2 x 32, or 4 x 26. This cell does not include casting gates, tray, or combs. * Sample throughput value assumes 1–2 combs per gel. † Sample throughput value assumes 1–4 combs per gel.

The Sub-Cell Model 192 cell offers even higher throughput capabilities than the Sub-Cell Model 96 cell and is also compatible with multichannel pipets. The cell accommodates gels up to 25 cm long. Using four or more 51-well analytical combs, more than two 96-well microplates of samples can be run. The longer gels and buffer recirculation ports of the Sub-Cell Model 192 cell make this cell ideal for Southern and northern blotting and separation of cosmid DNA restriction digests. The 25 x 10 cm and 25 x 15 cm trays, comb holder and combs, and electrode assemblies are interchangeable between the Sub-Cell Model 96 and Sub-Cell Model 192 electrophoresis cells.

All Sub-Cell Model 192 cells include a buffer tank, a safety lid with cables, a leveling bubble, and combs (26-well and 51-well). System configurations that include additional accessories are also available.

Cell size (W x L x H)	29 x 40 x 9 cm
Gel tray sizes (OD) (W x L)	25 x 10 cm
ReadyAgarose™ gels accommodated	No
Sample throughput	24–192
Base buffer volume	~3 L
Buffer recirculation	Yes
Bromophenol blue migration	~5.2 cm/hr (at 200 V)