UNOsphere™ Cation Exchange Media

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Overview

	UNOsphere™ Q	UNOsphere S
Type of ion exchanger	Strong anion	Strong cation
Functional group	-N⁺(CH₃)₃	-SO₃^-
Total ionic capacity	120 µeq/ml	280 µeq/ml
Dynamic binding capacity*
150 cm/hr	180 mg BSA/ml	60 mg IgG/ml
600 cm/hr	125 mg BSA/ml	30 mg IgG/ml
Shipping counterion	Cl^-	Na⁺
Median particle size	120 µm	80 µm
Recommended linear flow rate range**	50–1,200 cm/hr	50–1,200 cm/hr
Chemical stability
1.0 M NaOH (20°C)	Up to 10,000 hr	Up to 10,000 hr
1.0 M HCl (20°C)	Up to 200 hr	Up to 200 hr
Volume changes**
pH 4–10	<5%	<5%
0.01–1.0 M NaCl	<5%	<5%
pH stability	1–14	1–14
Autoclavability	Yes	Yes
Antimicrobial agent	20% ethanol	20% ethanol
Regeneration	1–2 M NaCl	1–2 M NaCl
Storage conditions	20% ethanol or 0.1 M NaOH	20% ethanol or 0.1 M NaOH

* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
** UNOshpere packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

UNOsphere ion exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

UNOsphere S support is used for strong cation exchange chromatography. UNOsphere Rapid S media builds on the properties and features of the UNOsphere family of supports with enhanced chemistries that overcome the pH shift that occurs with conductivity transitions.

Key Features

Efficient capture of biopharmaceutical molecules from crude feedstreams
Large pores that result in ultrahigh binding capacities at fast linear velocities
Hydrophilic polymeric beads engineered for high mechanical stability and low backpressures
Robust polymer designed to withstand repetitive clean-in-place cycles
Optimized to operate under 2 bar backpressure at velocities of 1,200 cm/hr
Stable to 1 M NaoH exposure to 10,000 hr
Availability in biopharmaceutical quantities
Fully supported for regulatory submissions