Micro Bio-Spin^® Columns with Bio-Gel^® P-30

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Overview

	Bio-Spin 6	Micro Bio-Spin 6	Bio-Spin 30	Micro Bio-Spin 30
Packed support	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-6 gel	Special-grade Bio-Gel P-30 gel	Special-grade Bio-Gel P-30 gel
Equilibration buffer	SSC-buffer*	10 mMTris, pH 7.4 or SSC buffer*	SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*
Applications	Desalting and buffer exchange	Desalting and buffer exchange	Desalting; nucleotide and small molecule removal	DNA sequencing reaction mixtures (Tris), and small molecule removal
Bed volume	1.1 ml	0.7 ml	1.1 ml	0.7 ml
Retention and recovery	90% recovery of 20 bases or bp, 99% retention of salts	90% recovery of 20 bases or bp, 99% retention of salts	95% recovery of 22 bases or bp, 98% retention of ddNTPs	95% recovery of 22% bases or bp, 98% retention of ddNTPs
MW exclusion limit, globular proteins	6,000	6,000	40,000	40,000
Sample volume	50–100 µl	10–75 µl	50–100 µl	10–75 µl
Centrifuge type	Swinging bucket	Microcentrifuge	Swinging bucket	Microcentrifuge
Autoclavability	Yes	Yes	Yes	Yes

*150 mM NaCl, 17.5 mM sodium citrate, pH 7.0

Column Selection Guide

	Bio-Spin 6	Micro Bio-Spin 6	Bio-Spin 30	Micro Bio-Spin 30	PCR Kleen
Packed support	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-6 gel	Special grade Bio-Gel P-30 gel	Special grade Bio-Gel P-30 gel	Special grade Size exclusion gel
Equilibration buffer	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, pH 7.4 or SSC buffer*	10 mM Tris, 1 mM EDTA, pH 7.0
Applications Desalting of oligonucleotides >20 bases	•	•	-	-	-
Labeling reactions: removal of unincorporated nucleotides >20 bases or bp from DNA	-	-	•	•	-
Removal of primers and primer-dimers from PCR products >200 bp	-	-	-	-	•
Buffer exchange (restriction fragments, PCR products, enzyme reactions, sequencing templates.)	•	•	-	•	-
DNA sequencing reaction mixture cleanup**	-	-	•	•	-
Riboprobe cleanup***	-	-	-	•	-
Desalting of antibody, enzyme, and protein solutions	•	•	-	•	-
Purification of proteins of moleular weight >6,000	•	•	-	-	-
Purification of proteins of molecular weight >40,000	-	-	•	•	-
Bed Volume	1.1 ml	0.7 ml	1.1 ml	0.7 ml	0.6 ml
Retention and recovery	90% recovery of 20 bases or bp, 99% retention of salts	90% recovery of 20 bases or bp, 99% retention of salts	95% recovery of 22 bases or bp, 98% retention of ddNTPs	95% recovery of 22 bases or bp, 98% retention of ddNTPs	85% recovery of ≥700bp, 95% retention of primers and primer-dimers
Molecular weight exclusion limit, globular proteins	6,000	6,000	40,000	40,000	8,000,000
Sample volume	50–100 µl	10–75 µl	50–100 µl	10–75 µl	25–100 µl
Centrifuge type	Swinging bucket	Microcentrifuge	Swinging bucket	Microcentrifuge	Microcentrifuge
Autoclavability	Yes	Yes	Yes	Yes	Yes

* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0
** In Tris buffer.
*** In RNase-free Tris buffer.

PCR and DNA Fragments

PCR Product and DNA Fragment Purification Guide

Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.

	Freeze 'N Squeeze™ DNA Gel Extraction Spin Columns	Micro Bio-Spin^® and Bio-Spin Columns	PCR Kleen™ Spin Columns
Application	Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels	Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting	Removal of PCR products >200 bp from other reaction mixture components
Starting material	≤700 mg gel slice	10–100 µl of sequencing reation mixture	25–100 µl of PCR or other enzyme reaction
Format	Spin-column extraction of frozen-thawed gel slices	Prepacked size exclusion spin column	Prepacked size exclusion spin column
Recovery	4–23 kb: >80% recovery; 0.5–4 kb:>70% recovery; 50–500 bp:>50% recovery	>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators	50–80%
Time required	<10 min	<10 min	<4 min

Description
Ordering

Bio-Spin and Micro Bio-Spin columns clean up DNA and protein samples quickly. Filled with specially sized Bio-Gel P gels, Bio-Spin and Micro Bio-Spin columns are shipped fully hydrated in Tris or SSC buffer and are ready to use. These products effectively clean up and remove salts, nucleotides, dye terminators, and small molecules from DNA, RNA, and protein samples.

Features include:

Convenience of prepacked, prehydrated gel support
Rapid sample cleanup with a 4 minute microcentrifuge spin
Efficient (98%) removal of ddNTPs and dye terminators
Improved yield — 95% recovery of DNA >22 bp
Sample loads from 10 to 100 µl
Safe riboprobe preparation with RNase-free Micro Bio-Spin P-30 Tris spin columns