Quantase™ Neonatal TGAL and Neonatal GALT Assays
The Bio-Rad Assay
- Not subject to the interferences commonly observed with fluorescent and bacterial inhibition methods
- Simplified protocols can be used on most plate readers
- Rapid results in under 2 hours (Total Galactose) and 3.5 hours (GALT)
Product Highlights
- Cost effective
- Easily automated
- Environmentally friendly, no flammables
- Rapid procedure
| Flexibility for Your Laboratory Needs |
| Total Galactose - Punch sample
- Add 5 µL elution reagent
- Incubate at RT for 30 minutes
- Vacuum transfer eluate
- Add 100 µL enzyme reagent
- Incubate at RT for 30 minutes
- Add 100 µL color reagent
- Incubate at RT for 2 minutes
- Read 570 nm and 690 nm
| TGALT (Transferase) - Punch sample
- Add 200 µL elution reagent
- Incubate at 37°C for 3 hours
- Transfer 120 µL
- Read 550 nm or 570 nm
- Add 50 µL enzyme reagent
- Incubate at RT for 10 minutes
- Read 550 nm or 570 nm
|
Clinical Background
Galactosemia is an inherited disorder of galactose metabolism, caused by a deficiency in one of three enzymes, the most important being galactose-1-phosphate uridyl transferase. Galactosemia may also be caused by a lack of galactokinase or UDP-galactose-4-epimerase activity.
Long-term consequences of galactosemia include mental retardation, developmental abnormalities, and even death. The worldwide prevalence of galactosemia is estimated to be 1:60,000–1:80,000. The Quantase Neonatal Total Galactose Screening Assay is an enzymatic colorimetric end-point method for the determination of total D(+) galactose in dried-blood spot specimens.
The Quantase Neonatal GALT Assay measures galactose-1-phosphate uridyl transferase activity in neonatal dried-blood spots to screen for galactosemia.
