Bio-Scale™ Mini Affi-Prep® Protein A Cartridges

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Overview

Capacities of Protein A Supports
Capacities of Protein A Supports
Immunoglobulin Affi-Gel Protein A
Capacity (mg/ml)		 Affi-Prep Protein A
Capacity (mg/ml)
Mouse lgG1 6–10 7–9
Mouse lgG2a 6–10 7–9
Mouse lgG2b 6–10 7–9
Mouse lgG3 6–10 7–9
Mouse lgM* 6–10 7–9
Human lgG 15 10–12
Sheep lgG 8–10 7–9
Cow lgG 8–10 7–9
Horse lgG 8–10 7–9
Goat lgG 8–10 7–9
Rabbit lgG 8–10 7–9
Dog lgG 8–10 7–9
Pig lgG 8–10 7–9

* Approximately 50% of all mouse lgMs bind using the MAPS buffer system.

Cartridge Selection by Application
Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Binding Capacity
Protein and Plasmid Purification
UNOSphere™ Rapid S media UNOSphere Rapid S Strong cation exchange SO3- Human IgG: 60 mg/ml*
CFT media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
Apatite purification kit Ceramic hydroxyapatite
Ceramic fluoropatite		 Hydroxyapatite
Fluoropatite		 (Ca5(PO4)3OH)2
Ca10(PO4)6F2		 Human IgG; 25-60 mg/ml**
Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media UNOSphere Rapid S Strong cation exchange SO3- Human IgG: 60 mg/ml*
CFT media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
Apatite purification kit Ceramic hydroxyapatite
Ceramic fluoropatite		 Hydroxyapatite
Fluoropatite		 (Ca5(PO4)3OH)2
Ca10(PO4)6F2		 Human IgG; 25–60 mg/ml**
Lysozyme 15–21 mg/g
Antibody Purification
CFT Media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
UNOsphere SUPrA™ media UNOsphere SUPrA Affinity Protein A  
Monoclonal Antibody Purification
mAb purification kit UNOsphere SUPrA
CHT™ Type 1
UNOsphere Q		 Affinity
Hydroxyapatite
Strong anion exchange		 Protein A
(Ca5(PO4)3OH)2
-N(CH3)3*		  
Human IgG: 25–60 mg/ml**
BSA* 180 mg/ml

* Flow rate = 150 cm/hr.
** Flow rate = 300 cm/hr.

Mini Specifications
Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
5 ml — 40 mm length x 12.6 mm inner diameter
10 ml — 80 mm length x 12.6 mm inner diameter**
50 ml — 80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Bio-Scale Mini Cartridge Selection Guide
Bio-Scale™ Mini Cartridge Selection Guide
Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
5 x 1 ml1 x 5 ml5 x 5 ml
Protein and Plasmid Purification
High QMacro-Prep® High QStrong anion exchange–N+(CH3)3≥37 mg BSA732-4120732-4122732-4124
UNOsphere™ QUNOsphereQStrong anion exchange–N+(CH3)3180 mg BSA732-4100732-4102732-4104
UNOsphere SUNOsphere SStrong cation exchange–SO3-60 mg human lgG732-4110732-4112732-4114
DEAEMacro-Prep DEAEWeak anion exchange–HN+(C2H5)2>35 mg protein732-4140732-4142732-4144
Protein Purification
High SMacro-Prep High SStrong cation exchange–SO3-≥49 mg human lgG732-4130732-4132732-4134
IgG Purification
DEAE BlueDEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

—
732-4632732-4634
Antibody Purification
Protein AUNOsphere SUPrA™AffinityRecombinant protein A30 mg/ml human IgG
732-4200, 1 x 1 ml732-4201, 5 x 1 ml732-4202, 1 x 5 ml
Protein AAffi-Prep®
protein A		AffinityProtein A8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG		732-4600732-4602
—
CHT Type ICHT™ ceramic
hydroxyapatite
Type I, 40 µm		Hydroxyapatite(Ca5(PO4)3OH)225–60 mg human lgG
—
732-4322732-4324
CHT Type IICHT ceramic
hydroxyapatite
Type II, 40 µm		Hydroxyapatite(Ca5(PO4)3OH)215–25 mg human lgG
—
732-4332732-4334
Affinity-Tagged Protein Purification
IMACProfinity™ IMACAffinityIminodiacetic acid≥15 mg recombinant
histidine (His)-tagged protein		732-4610732-4612732-4614
GSTProfinity GSTAffinityGlutathione≥10 mg/ml recombinant
GST-tagged protein		732-4620732-4622732-4624
Purification of Serum Proteins, Enzymes
BlueAffi-Gel BlueAffinityCibacron Blue
F3GA		0.2 ml serum
11 mg albumin
—
732-4642732-4644

Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
1 x 5 ml5 x 5 ml5 x 10 ml1 x 50 ml5 x 50 ml
Desalting, Buffer Exchange
P-6Bio-Gel® P-6Gel filtrationPolyacrylamide10–22% CV**732-
4502		732-
4504		732-
5304		732-
5312		732-
5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

PDF Bulletin 5584 To read more click here

Protein Coupling Comparison
Protein Coupling Comparison

Protein coupling with Affi-Gel 10 and Affi-Gel 15 gels. Coupling conditions: Each protein solution (3 ml of 0.1 M MOPS, pH 7.5, containing 40 mg protein) was combined with 2 ml of Affi-Gel gel.

Bio-Scale Mini Affi-Prep protein A cartridges are 1 ml and 5 ml cartridges filled with Affi-Prep protein A support. Affi-Prep protein A support can be used to yield highly purified immunoglobulins (IgGs), selectively remove IgG prior to analysis of other immunoglobulin classes, or adsorb immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. The cartridges can be used for method scouting or simple purifications.

Advantages include:

  • Binds a wide range of monoclonal and polyclonal antibodies, including mouse IgG1
  • Macroporous polymeric support is not compressible under normal operating conditions
  • Compatible with most commonly used chromatographic buffers
  • Stable pH range from 2–10
  • Multiple chromatographic cycles without loss of capacity
  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer
  • Easy to use

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

For more information, request bulletins 5326 and 5584.

* U.S. Patent 7,208,087.

Bio-Scale Mini Affi-Prep Protein A Cartridges

732-4600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $600.00
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Bio-Scale Mini Affi-Prep Protein A Cartridge

732-4602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $505.00
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Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download [ Add to Cart ]
5326 Chromatography Column Connection Guide, Rev A Click to download [ Add to Cart ]
5701 Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B Click to download [ Add to Cart ]
5726 Profinia Protein A and G methods Product Information Sheet, Rev A Click to download [ Add to Cart ]
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download [ Add to Cart ]
LIT230 Instruction Manual, Affi-Prep Protein A Matrix Click to download [ Add to Cart ]
LIT79 Instruction Manual, Affi-Prep Protein A MAPS II Kit Click to download [ Add to Cart ]
10006546 Bio-Scale Mini Affi-Prep Protein A Cartridges, 1 and 5 ml, Instruction Manual, Rev B Click to download