Nuvia™ Q Media

print email

Overview

Introducing Nuvia™ Q Media

Nuvia media are ultra-high binding capacity, next-generation ion exchangers designed to meet current and future process needs. Nuvia Q, our newest anion exchange media, delivers excellent resolution properties for polishing applications in downstream processes.

Get a taste of high-capacity performance with Nuvia Q and Nuvia S media.
Visit request a sample.

High Capacity

High Capacity at Fast Flow Rates

Nuvia™ Q media is designed to meet the demanding pressure-flow requirements of downstream processes. With strong mechanical properties and optimized bead size distribution, Nuvia Q media consistently delivers high binding capacity at fast linear flow velocities, allowing users the advantage of increased productivity without compromising binding capacity.

Dynamic binding capacity vs. flow velocity of Nuvia Q media. Each 1.1 cm column was packed to a 10.6 cm bed height with Nuvia Q, agarose Q, or polymeric Q media. Five mg/ml BSA in 20 mM Tris-HCl, pH 8.5, was loaded onto each column until 10% breakthrough was observed. DBC BSA, dynamic binding capacity bovine serum albumin; BT, breakthrough.

For more information about Nuvia Q media, see bulletin 6129.

Tutorial: Nuvia™ Ion Exchangers

Nuvia™ Q and S Ultra-High Capacity Ion Exchange Media

Bio-Rad now offers two ultra-high capacity ion exchangers: Nuvia S, which can be used for capture and/or intermediate polishing, and Nuvia Q, which can be used for intermediate and/or final polishing.

Baseline Resolution

Separation of Proteins with Similar pIs

Purification of whey probein on Nuvia Q Media

Purification of whey protein on Nuvia™ Q media. Crude whey feed stock (8 mg) was loaded on a 7 x 27 mm column in 0.02 M sodium phosphate buffer, pH 6.0. The samples were eluted using a segmented gradient of 0-0.1 M NaCl, 0.1–0.3 M NaCl, and 0.3–0.9 M NaCl. Each fraction was 2 ml and analyzed by A₂₈₀.

Nuvia Q media exhibits excellent baseline resolution of biomolecules with similar isoelectric points (pIs). The figure shows the purification of α-lactalbumin (ALA, pI: 4.7–5.1; Pool 1) and β-lactoglobulin (BLG, pI: 5.2; Pool 2), the major proteins of whey, accounting for approximately 75% of the total mass of crude whey. The two major peaks were resolved using a segmented NaCl gradient. Purities of the two purified proteins were estimated by densitometry to be at least 85%.

For more information about Nuvia Q media, see bulletin 6128.

Nuvia Q is an ultra-high capacity, next-generation anion exchange media. Nuvia Q media delivers high binding capacity over a range of pH and flow rates, providing a wide experimental design space for process developers. This exceptional binding capacity makes Nuvia Q the anion exchanger of choice for biopharmaceuticals as upstream processes deliver higher concentrations of feed titers. Along with high binding capacity, Nuvia Q media also delivers excellent performance for polishing applications. Nuvia Q media can significantly improve productivity while contributing to reduced capital costs, space requirements, and cycle time for downstream biotherapeutic purification.

Nuvia Q media delivers value and flexibility by providing users:

Best-in-class binding capacity at fast flow rates
Rapid mass transfer kinetics
Efficient resolution of biomolecules from crude feedstreams
Robust media design providing competitive NaOH stability
Full regulatory support

Type of ion exchanger	Strong anion
Functional Group	–N(CH₃)₃⁺
Total ionic capacity	100–170 µeq/ml
Dynamic binding capacity*: 300 cm/hr	≥170 mg/ml
Shipping counterion	CI^–
Median particle size	85 ± 15 µm
Operational flow rate**	50–600 cm/hr
Chemical stability: 1.0 N NaOH (20°C)	Up to 1 week
Chemical stability: 0.01 N NaOH (20°C)***	Up to 5 years
Gel bed compression ratio	1.10–1.15
pH stability short-term***	2-14
pH stability long-term***	4–12
Shipping Solution	20% ethanol + 1 M NaCl
Regeneration	1–2 M NaCl
Sanitation	0.5–1.0 N NaOH
Storage conditions	20% ethanol or 0.01 N NaOH
* 10% breakthrough capacity determined with 5 mg/ml BSA in 20 mM Tris-HCI, pH 8.5.
** Recommended elution flow rates are ≤ 150 cm/hr to minimize viscosity-induced backpressure.
*** Data derived under accelerated conditions at 60°C.