Bio-Scale™ Mini Profinity eXact™ Cartridges

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Overview

Sizes	1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter 50 ml — 80 mm length x 28.2 mm inner diameter
* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

To read more click here

Cartridge Comparison

Cartridge Comparison on Profinia System

Comparison of Bio-Scale Mini Cartridges to other affinity columns on the Profinia Protein Purification system. Yield (A) and purity (B) of various proteins purified using either Bio-Scale Mini Profinity IMAC cartridges () or commercially available prepacked columns HisTrap FF () or HisTrap HP () on the Profinia system. Rec1, 75 kD; Fih1, 37 kD; DHFR, 25 kD; SME1, 11 kD.

To read more click here

Cartridge Purification

Profinity eXact Cartridge Purification

Profinity eXact cartridge purification. SDS-PAGE analysis of a purification of Profinity eXact fusions to a 26 kD Green Fluorescent Protein (GFP) and to a 40 kD maltose binding protein (MBP). Crude E. coli lysate containing the expressed proteins (2 ml) was loaded onto a Bio-Scale Mini Profinity eXact 1 ml cartridge on a BioLogic DuoFlow system with bind/wash buffer at 1 ml/min. The cartridge was washed with 10 column volumes (CV) of the same buffer at 1 ml/min. Proteins were eluted with 3 CV sodium fluoride buffer at room temperature and at 0.1 ml/min for 30 min. Total purification time required to generate the tag-free proteins without addition of protease was approximately 60 min. MW, molecular weight markers; L, load (crude lysate); FT, flowthrough; W, wash; E, eluate. Yellow arrows indicate the tagged proteins; red arrows indicate the tag-free purified proteins.

Resin Stability and Reusability

Profinity eXact Resin Stability and Reusability

Stability and reusability of Profinity eXact purification resin. A, overlaid chromatograms of MBP purification with a 1 ml Bio-Scale Mini Profinity eXact cartridge on the BioLogic DuoFlow system. Binding capacities for cycles 1 to 5 ranged from 3.7 mg/ml to 3.3 mg/ml; B, SDS-PAGE analysis of MBP protein eluted from cartridge cycling studies using Criterion 4–20% Tris-HCl gels. Lane 1, E. coli lysate containing tagged MBP protein; lane 2, Precision Plus Protein standards; lanes 3–7, 3 µg of tag-free MBP protein eluted from cartridge cycling studies; lane 8, Precision Plus Protein standards. Percentage purity of proteins in lanes 3–7 is indicated.

Bio-Scale Mini Profinity eXact cartridges are 1 and 5 ml cartridges filled with Profinity eXact purification resin. Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The cartridges are used for on-column purification and cleavage of recombinant proteins using the Profinity eXact fusion-tag system. The novel Profinity eXact fusion-tag system generates a highly purified recombinant protein containing only its native amino acid sequence in a single step and in a fraction of the time of other methods.

Profinity eXact benefits:

Purification and processing of fusion-tagged proteins in a single step
On-column cleavage in as little as 30 minutes
No protease addition required
Precise cleavage at N-terminus to generate native protein sequence

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, the Profina protein purification system, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (see bulletin 5326).

For more information, request bulletin 5655.

*U.S. patent 7,208,087.

Bio-Scale Mini Profinity eXact Cartridges

732-4646
Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

List Price:		$180.00
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Bio-Scale Mini Profinity eXact Cartridges

732-4647
Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

List Price:		$340.00
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Bio-Scale Mini Profinity eXact Cartridge

732-4648
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

List Price:		$340.00
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Quantity:		Add to Cart Check Out
Add to Hot List		Add to Quote Get Quote

Number	Description	Options
5584	Bio-Scale Mini Cartridges, Product Information Sheet, Rev B	Click to download	[ Add to Cart ] Check Out
10011164	Bio-Scale Mini Profinity eXact Cartridges Manual, Rev B	Click to download
5655	Profinity eXact Resin Product Information Sheet, Rev A	Click to download	[ Add to Cart ] Check Out
5326	Chromatography Column Connection Guide, Rev A	Click to download	[ Add to Cart ] Check Out
5725	Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A	Click to download	[ Add to Cart ] Check Out
5742	Profinity eXact Purification Methods on the Profinia System, Rev. A	Click to download	[ Add to Cart ] Check Out
5668	Profinity eXact Fusion-Tag System Brochure, Rev A	Click to download	[ Add to Cart ] Check Out
5646	Profinity eXact Cloning and Expression Kits, PIS, Rev B	Click to download	[ Add to Cart ] Check Out
5656	Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B	Click to download	[ Add to Cart ] Check Out
5652	Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A	Click to download	[ Add to Cart ] Check Out
10011162	Profinity eXact Tag Antibody	Click to download
10011163	Profinity eXact Mini Spin Columns Manual, Rev A	Click to download	[ Add to Cart ] Check Out
5766	Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A	Click to download	[ Add to Cart ] Check Out
10011260	Profinity eXact Protein Purification System Manual, Rev A	Click to download	[ Add to Cart ] Check Out

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