Bio-Scale™ Mini CHT™ Ceramic Hydroxyapatite Cartridges

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Overview

Bio-Scale Mini Cartridge Specifications


Sizes	1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter 50 ml — 80 mm length x 28.2 mm inner diameter

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

To read more click here

mAb Workflow

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

Cartridge Selection

Prepacked Cartridge Selection by Application

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	-N⁺(CH₃)₃	≥37 mg BSA
UNOsphere™ Q	UNOsphere Q	Strong anion exchange	-N⁺(CH₃)₃	180 mg BSA
UNOsphere™ S	UNOsphere S	Strong cation exchange	-SO₂^-	60 mg human IgG
DEAE	Macro-Prep^® High DEAE	Weak anion exchange	-HN⁺(C₂H₅)₂	≥35 mg protein
Protein Purification
High S	Macro-Prep^® High S	Strong cation exchange	-SO₂^-	≥49 mg human IgG
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum
Antibody Purification
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal IgG, 16–23 mg human IgG
UNOsphere SUPrA™ Affinity media	UNOsphere™	Affinity	Recombinant Protein A	30 ± 3 mg/ml at 150 cm/hr 25 ± 2 mg/ml at 300 cm/hr 20 ± 2 mg/ml at 450 cm/hr
CHT Type I	CHT ceramic Hydroxyapitite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg Human IgG
CHT Type II	CHT ceramic Hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human IgG
Affinity-Tagged Protein Purification
IMAC**	Profinity IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant His-tagged protein
GST**	Profinity GST	Affinity	Glutathione	≥10mg/ml recombinant GST-tagged protein
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel Filtration	Polyacrylamide	10–22% CV***
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

CHT Specifications

	Type I	Type II
Functional groups	Ca²⁺, PO₄, OH	Ca²⁺, PO₄, OH
Observed dynamic binding capacity lysozyme (Lys)	≥25 mg Lys/g CHT	≥12.5 mg Lys/g CHT
Nominal pore diameter	600–800 A	800–1,000 A
Maximum backpressure	100 bar (1,500 psi)	100 bar (1,500 psi)
Nominal mean particle size	20 ± 2, 40 ± 4, and 80 ± 8 µm	20 ± 2, 40 ± 4, and 80 ± 8 µm
Bulk density	0.63 g/ml	0.63 g/ml

CHT Character

CHT Characteristics

	Type I	Type II
Observed dynamic binding capacity IgG	25–60 mg IgG/ml CHT*	15–25 mg IgG/ml CHT**
Typical linear flow rate range	50–1,000 cm/hr
pH Stability	6.5–14 pH
Base Stability	at least 21 months in 1 N NaOH
Regeneration	500 mM sodium phosphate, pH7 1,000 mM trisodium phosphate, pH 11–12
Autoclavability (bulk)	121°C, 20 min in phosphate buffer, pH 7
Sanitization	1–2 N NaOH
Recommended column storage	0.1 M NaOH
Shelf life (dry, unused material)	85 months stored dry, sealed, and at room temperature

* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5
** 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5

IgG Protocol

Purification of IgG using CHT

Purification of IgG Using CHT

Purification of IgG using CHT Ceramic Hydroxyapatite.

To read more click here

Acidic Proteins Protocol

Acidic Proteins Protocol Using CHT

Acidic Proteins Protocol using CHT
Optimizing the bioprocess requires management of multiple variables ranging from matrix interaction, elution characteristics, scale-up from bench, regulation and drug safety requirements, and process robustness and economics. This protocol has been developed as general starting guidelines for the purification of most proteins and nucleic acids and may help to reduce time spent in methodology development.

Bio-Scale Mini CHT cartridges are 5 ml cartridges filled with CHT ceramic hydroxyapatite support, a leading purification medium for today's demanding downstream process industry. The mixed-mode support offers unique selectivities and often separates biomolecules that appear homogeneous using other chromatographic methods. The diverse binding capabilities of CHT for host cell proteins, leached protein A, antibody dimers and aggregates, nucleic acids, and viruses allow its use at any stage from initial capture to final polishing.

The cartridges are available with either CHT Type I or Type II, 40 µm media. The Type I support has a protein binding capacity of 25–60 mg of human IgG/ml and higher capacity for acidic proteins. Type II has a protein binding capacity of 15–25 mg of human IgG/ml and gives better resolution of nucleic acids and of proteins that elute early. Type II has very low affinity for albumin, so it is often more suitable than Type I for purification of many species and classes of immunoglobulins.

The robust properties of CHT ceramic hydroxyapatite improve efficiency, yield, and financial value through:

Excellent capture at elevated flow rates, enabling processing at all scales
Large capacity for high-titer upstream feedstocks
Exceptional selectivity, allowing a two-step chromatographic process

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (request bulletin 5326).

CHT Ceramic Hydroxyapatite Mechanism

Hydroxyapatite contains two types of binding sites, positively charged calcium and negatively charged phosphate groups. These sites are distributed regularly throughout the crystal structure of the matrix. Solute species dominantly interact through cation exchange via the phosphate groups and/or metal affinity via the calcium atoms.

For more information, request bulletin 5709.

*U.S. patent 7,208,087.

Bio-Scale Mini CHT Type I Cartridge

732-4322
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

List Price:		$140.00
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Bio-Scale Mini CHT Type I Cartridges

732-4324
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type I

List Price:		$560.00
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Bio-Scale Mini CHT Type II Cartridge

732-4332
Pkg of 1, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

List Price:		$140.00
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Bio-Scale Mini CHT Type II Cartridges

732-4334
Pkg of 5, 5 ml, prepacked, CHT ceramic hydroxyapatite multimodal chromatography media cartridge, Type II

List Price:		$560.00
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Number	Description	Options
10011433	Bio-Scale Mini CHT Ceramic Hydroxyapatite Cartridges, 5 ml, Instruction Manual, Rev A	Click to download	[ Add to Cart ] Check Out
5584	Bio-Scale Mini Cartridges, Product Information Sheet, Rev B	Click to download	[ Add to Cart ] Check Out
5326	Chromatography Column Connection Guide, Rev A	Click to download	[ Add to Cart ] Check Out
5709	How CHT Ceramic Hydroxyapatite Works, Rev A	Click to download	[ Add to Cart ] Check Out
5667	CHT Ceramic Hydroxyapatite, Rev A	Click to download	[ Add to Cart ] Check Out
2156	CHT Ceramic Hydroxyapatite — A New Dimension in Chromatography of Biological Molecules, Rev C	Click to download	[ Add to Cart ] Check Out
5739	Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev B	Click to download
Lit-611	Instruction Manual, CHT Ceramic Hydroxyapatite, Rev E	Click to download	[ Add to Cart ] Check Out
2731	Plasmid Purification Using CHT Ceramic Hydroxyapatite Support, Rev A	Click to download	[ Add to Cart ] Check Out
2780	Purification of Murine IgG₁ Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A	Click to download	[ Add to Cart ] Check Out
2524	Purification of Horse IgG_T Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C	Click to download
2774	Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A	Click to download
1986	Effect of pH on Gradient Elution of Proteins on Two Types of CHT Ceramic Hydroxyapatite, Rev B	Click to download	[ Add to Cart ] Check Out
1985	Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.	Click to download	[ Add to Cart ] Check Out
2940	Removal of Aggregate From an IgG₄ Product Using CHT Ceramic Hydroxyapatite, Rev A	Click to download
2849	Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A	Click to download
2535	Purification of Recombinant Human alpha 1-Antitrypsin From Transgenic Milk Using CHT Ceramic Hydroxyapatite, Rev B	Click to download
2575	Purification of a Calcium-Binding Protein Using CHT Ceramic Hydroxyapatite	Click to download
2946	Plasmid DNA Adsorption Behavior on CHT Ceramic Hydroxyapatite Type II Support, Rev A	Click to download
5662	Process Chromatography Applications Laboratory Brochure, Rev B	Click to download	[ Add to Cart ] Check Out

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