Bio-Scale™ Mini Macro-Prep® High S Cartridges

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Overview

Specifications
Specifications
Property DEAE High Q High S CM
Type of support Weak anion Strong anion Strong cation Weak cation
Functional ligand –N+(C2H5)2 –N+(CH3)3 –SO3- –COO-
Ionic capacity 175 ±75 µeq/ml 400 ±75 µeq/ml 160 ±40 µeq/ml 210 ±40 µeq/ml
Typical binding capacity 35 mg BSA/ml 40 mg BSA/ml 70 mg IgG/ml 35 mg hemoglobin/ml
Counterion Cl- Cl- Na+ Na+
Nominal particle size 50 µm 50 µm 50 µm 50 µm
Nominal pore size 1,000 Å 1,000 Å 1,000 Å 1,000 Å
Recommended maximum linear flow rate 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr 3,000 cm/hr
Chemical stability        
   1% SDS, 24 hr Yes Yes Yes Yes
   6 M guanidine-HCl, 24 hr Yes Yes Yes Yes
Volume changes        
   pH 4–10 <1% <1% <3% <1%
   0.1–1.0 M NaCl <5% <5% <9% <4%
Autoclavability* (121°C, 80 min) Yes Yes Yes Yes
pH stability 1–10 1–10 1–12 1–12
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol 20% ethanol
Regeneration 1 M NaCl 1 M NaCl 1 M NaCl 1 M NaCl

* Do not autoclave the OH- form.

Cartridge Selection by Application
Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Binding Capacity
Protein and Plasmid Purification
UNOSphere Rapid S media UNOSphere Rapid S Strong cation exchange SO3- * Human IgG: 60 mg/ml
CFT media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
Apatite purification kit Ceramic hydroxyapatite
Ceramic fluoropatite		 Hydroxyapatite
Fluoropatite		 (Ca5(PO4)3OH)2
Ca10(PO4)6F2		 ** Human IgG; 25-60 mg/ml
Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media UNOSphere Rapid S Strong cation exchange SO3- * Human IgG: 60 mg/ml
CFT media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
Apatite purification kit Ceramic hydroxyapatite
Ceramic fluoropatite		 Hydroxyapatite
Fluoropatite		 (Ca5(PO4)3OH)2
Ca10(PO4)6F2		 ** Human IgG; 25–60 mg/ml
Lysozyme 15–21 mg/g
Antibody Purification
CFT Media Ceramic fluoropatite Fluoropatite Ca10(PO4)6F2 Lysozyme 15–21 mg/g
UNOsphere SUPrA media UNOsphere SUPrA Affinity Protein A  
Monoclonal Antibody Purification
mAb purification kit UNOsphere SUPrA
CHT Type 1
UNOsphere Q		 Affinity
Hydroxyapatite
Strong anion exchange		 Protein A
(Ca5(PO4)3OH)2
-N(CH3)3*		  
** Human IgG: 25–60 mg/ml
* BSA 180mg/ml

* Flow rate = 150 cm/hr; ** flow rate = 300 cm/hr

Mini Cartridge Specifications
Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
5 ml — 40 mm length x 12.6 mm inner diameter
10 ml — 80 mm length x 12.6 mm inner diameter**
50 ml — 80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Bio-Scale Mini Cartridge Selection Guide
Bio-Scale™ Mini Cartridge Selection Guide
Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
5 x 1 ml1 x 5 ml5 x 5 ml
Protein and Plasmid Purification
High QMacro-Prep® High QStrong anion exchange–N+(CH3)3≥37 mg BSA732-4120732-4122732-4124
UNOsphere™ QUNOsphereQStrong anion exchange–N+(CH3)3180 mg BSA732-4100732-4102732-4104
UNOsphere SUNOsphere SStrong cation exchange–SO3-60 mg human lgG732-4110732-4112732-4114
DEAEMacro-Prep DEAEWeak anion exchange–HN+(C2H5)2>35 mg protein732-4140732-4142732-4144
Protein Purification
High SMacro-Prep High SStrong cation exchange–SO3-≥49 mg human lgG732-4130732-4132732-4134
IgG Purification
DEAE BlueDEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

—
732-4632732-4634
Antibody Purification
Protein AUNOsphere SUPrA™AffinityRecombinant protein A30 mg/ml human IgG
732-4200, 1 x 1 ml732-4201, 5 x 1 ml732-4202, 1 x 5 ml
Protein AAffi-Prep®
protein A		AffinityProtein A8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG		732-4600732-4602
—
CHT Type ICHT™ ceramic
hydroxyapatite
Type I, 40 µm		Hydroxyapatite(Ca5(PO4)3OH)225–60 mg human lgG
—
732-4322732-4324
CHT Type IICHT ceramic
hydroxyapatite
Type II, 40 µm		Hydroxyapatite(Ca5(PO4)3OH)215–25 mg human lgG
—
732-4332732-4334
Affinity-Tagged Protein Purification
IMACProfinity™ IMACAffinityIminodiacetic acid≥15 mg recombinant
histidine (His)-tagged protein		732-4610732-4612732-4614
GSTProfinity GSTAffinityGlutathione≥10 mg/ml recombinant
GST-tagged protein		732-4620732-4622732-4624
Purification of Serum Proteins, Enzymes
BlueAffi-Gel BlueAffinityCibacron Blue
F3GA		0.2 ml serum
11 mg albumin
—
732-4642732-4644

Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
1 x 5 ml5 x 5 ml5 x 10 ml1 x 50 ml5 x 50 ml
Desalting, Buffer Exchange
P-6Bio-Gel® P-6Gel filtrationPolyacrylamide10–22% CV**732-
4502		732-
4504		732-
5304		732-
5312		732-
5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

PDF Bulletin 5584 To read more click here

Bio-Scale Mini Macro-Prep High S cartridges are available in 1 and 5 ml sizes. These cartridges come prepacked with Macro-Prep High S strong cation exchange media and are ideal for method scouting and small-scale purifications.

Macro-Prep High S media contain sulfonate functional groups and are ideal for purifying basic and neutral proteins and peptides. The support is an excellent choice for rapid purification and meets the demands of analytical, semipreparative, and process-scale applications. This methacrylate copolymer bead provides high-resolution separations at very high flow rates. Changes in pH or ionic strength of the buffer do not cause shrinking or swelling of the support. Macro-Prep High S can be sanitized with chaotropic agents and can be autoclaved. It provides consistent and reproducible results.

Advantages of Macro-Prep High S include:

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

 

Bio-Scale Mini cartridges have a polypropylene double-wall construction* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Bio-Scale Mini cartridges are ideal for low-pressure chromatography applications. Luer fittings make connections simple; adaptors are available to allow connection to all types of chromatography systems.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

* U.S. Patent 7,208,087.

Bio-Scale Mini Macro-Prep High S Cartridges

732-4130
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $190.00
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Bio-Scale Mini Macro-Prep High S Cartridge

732-4132
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $75.00
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Bio-Scale Mini Macro-Prep High S Cartridges

732-4134
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $265.00
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Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download [ Add to Cart ]
10006400 Bio-Scale Mini Macro-Prep High Q, High S, and DEAE Cartridges, 1 and 5 ml, Instruction Manual, Rev B Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download [ Add to Cart ]
5643 Macro-Prep High S Support Product Information Sheet, Rev A. Click to download [ Add to Cart ]
1985 Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B. Click to download [ Add to Cart ]
1913 Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B Click to download
LIT271 Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D Click to download
2026 Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal Antibodies Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download [ Add to Cart ]
2079 Non-Protein A Based Purification of MAb 414, Rev B Click to download